Protein Labeling Reagents
Cayman Chemical Fluorescein 5 6-isothiocyana
An amine-reactive fluorescent probe; reacts with amine groups to form conjugates with proteins for detection by various fluorescent-based applications; ex/em = 485/535 nm, respectively
eMolecules 198978-94-8 | Medchem Express | 5(6)-ROX | 5mg | 415688918 | HY-D0043 | MFCD29912920 | 1069.224 | C66H60N4O10
Ambeed | 4-Chloro-2-fluorophenol | 5g | 552698895 | A300724 | 348-62-9 | MFCD00075030 | 146.550 | C6H4ClFO
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Biotium CF488A-Dextran 10, 000 MW, Anionic and Fixable
CF dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran conjugates are available in a variety of molecular weights, and contain a formaldehyde-fixable free-amine group. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
![Aat Bioquest Tide Quencher™ 3 maleimide [TQ3 maleimide]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/501953104.JPG-250.jpg)
Aat Bioquest Tide Quencher™ 3 maleimide [TQ3 maleimide]
TQ3 is designed to be a superior quencher to TAMRA, TF3 and Cy3. TQ3 has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides.
Biotium 5-TAMRA-PEO3-amine
5-TAMRA-PEO3-amine (Tetramethylrhodamine-PEO3-amine) has a long water-soluble PEG spacer separating the dye and the amine functional group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides. Properties: Ex/Em (MeOH) = 544/571 nm; Dark purple solid soluble in DMSO, DMF, or H2O; Store at 4°C and protect from light; C 37 H 42 F 6 N 4 O 11; MW: 832.74
Biotium Fura-2, Pentapotassium Salt
Fura-2, pentaammonium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2-free) to 335 nm (Ca2-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm.Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C29H22K5N3O14; MW: 832; [113694-64-7].
Biotium 5-(and-6-)-((N-(5-aminopentyl)amino)carbonyl) Tetramethylrhodamine (Tetramethylrhodamine cadaverine) (mixed isomer)
INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins. Properties: Light yellow solid soluble in DMSO or DMF; Store at 4°C and protect from light; C30H34N4O4; MW: 295.08.
Biotium CF594 Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 dye has excitation/emission at 593/614 nm.
Biotium Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF594 Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP goat anti-rabbit CF594 dye tyramide. The dye has an excitation/emission of 593/614 nm. The kit contains sufficient reagent for 50-150 slides.
Biotium Phalloidin, CF488A, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Freen fluorescent CF488A has excitation/emission at 490/515 nm.
Bioworld Agarose Gel Loading Dye (6X) Glycerol with BB, 25 mL
Gel loading dye for use in agarose gel electrophoresis for molecular biology Prepared in sterile 3D water., Store at RT.Technical Information:6X concentrationBromophenol blue (0.25%)Xylene cyanol FF (0.25%)Glycerol in water (30%)Storage: 2-8C
Biotium Mix-n-Stain™ CF488A Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm. It is suitable for labeling ligands for cell surface staining.