Protein Labeling Reagents
Biocare Medical, LLC Background Sniper - 100 ml
Background Sniper is specifically formulated for superior pH stability and is sodium azide and thimerosal free. It is a universal blocking reagent used for reducing nonspecific background staining often found with immunohistochemistry, ELISA, immunoelectron microscopy and immunogold techniques. Casein has proven to be a superior blocking reagent as compared to serum proteins. It can also be used on an automated staining system.
STEMCELL Technologies GloCell™ Fixable Viability Dye Violet 540, Size: 100 Tests
GloCell™ Fixable Viability Dye Violet 540 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 540 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 540 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
LICOR IRDye 680RD DBCO Infrared Dye (5 mg)
IRDye 680RD DBCO provides the functionality for preferential labeling of molecules that contain azide groups (–N3) with IRDye 680RD.
LICOR IRDye 800CW Alkyne Infrared Dye (5mg)
IRDye 800CW Alkyne provides the functionality for preferential labeling of molecules containing azide groups (–N3) with IRDye 800CW.
Abcam Rhodol, 5MG
MW 331.3 Da, Purity >95%. Highly fluorescent dye useful for labeling oligonucleotide, antibody and protein studies and for the preparation of peptide and esterase substrates. Rhodol is a structural hybrid of fluorescein and rhodamine. It retains the pH-sensitive properties conferred by a phenolic hydroxyl group, but the amine substituent reduces the pKa values. Rhodol exhibits a high extinction coefficient (~70,000 cm-1M-1) and high quantum yield (~0.8-0.9) in aqueous solution, with absorption maxima at approximately 490 nm and emission maxima between 520 and 545 nm. As with fluorescein-based dyes, the fluorescence excitation profiles of rhodol-based dyes are pH dependent, which can be useful for ratiometric pH determinations intracellularly.
The product is subject to the following: Abcam Restricted Use Statement
Biotium Phalloidin, CF405M, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF405M has excitation/emission at 408/452 nm.
Biotium Phalloidin, CF568, 50 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 has excitation/emission at 562/583 nm.
Biotium Phalloidin, CF350, 50 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable, blue fluorescent CF350 has excitation/emission at 347/448 nm.
Biotium Fluorescein Methotrexate, Triammonium Salt, 1mg
Fluorescein methotrexate is useful for studying anti-metabolite resistance and spontaneous gene amplification. The dye is actively taken up by cells and binds to dihydrofolate reductase (DHFR) quantitatively.Properties: Ex/Em nm 496/516 nm; Yellow solid soluble in pH>6.5 buffer, DMF or DMSO; Store desiccated at 4°C and protect from light; C46H54N14O9S; MW:979.08
Biotium Phalloidin, CF640R, 50 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R has excitation/emission at 642/662 nm.
Biotium Phalloidin, CF633, 50 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 has excitation/emission at 630/650 nm.
Biotium Mix-n-Stain™ CF405M Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF405M dye has excitation/emission at 408/452 nm. It is suitable for labeling ligands for cell surface staining. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium MemBrite™ Fix 594/615 Cell Surface Staining Kit, 100 Reactions
MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant, or CellMask™. This trial size kit has reagents sufficient for 100 labeling reactions. The kit contains 1 vial of MemBrite™ Fix 594/615 that is sufficient for 20 uL of 1000X dye after DMSO reconstitution.