Protein Labeling Reagents
Biotium DNP Tyramide
DNP tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.
Bioworld Glow Cyanoblue Dye, 5 mL
Premixed and ready-to-load gel dyes. Glow Loading Dyes generate intense DNA/RNA bands with little background or band distortion and can be used with any type of agarose or acrylamide gels. Glow CyanoBlue dye contains bromophenol blue and xylene cyanol in a ficoll-based solution with ethidium bromide (6X concentration).
Vector Laboratories Wheat Germ Agglutinin (WGA) Fluorescein-labeled 5 mg
Wheat germ agglutinin (WGA) contains a group of closely related isolectins that bind N-acetylglucosamine (preferentially the dimers and trimers of this sugar) and oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA also interacts with some glycoproteins via sialic acid residues (see succinylated WGA). This fluorescein-labeled WGA features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution.
![Aat Bioquest 5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-250.jpg)
Aat Bioquest 5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*
Tetramethylrhodamine-5-(and-6)-maleimide is an excellent reagent for preparing orange fluorescent bioconjugates through thiol modifications of peptides, nucleotides, nucleic acids and proteins.
Biotium Fluo-3, AM Ester, Special Packag, 20x50ug
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening. Properties: Orange red solid soluble in DMSO; Store -20°C and protect from light, especially when in solution; C51H50Cl2N2O23; MW: 1129.9; [121714-22-5]
Aat Bioquest 7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*
This compound is used as a calibration standard for 7-hydroxy-4-methylcoumarin-based enzyme substrates. It has pH-dependent fluorescence.
Medchemexpress LLC HY-D0048 10mg Medchemexpress, 5-TAMRA-SE CAS:150810-68-7 Purity:>98%
Medchemexpress, HY-D0048 10mg 5-TAMRA-SE CAS:150810-68-7 0 Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Biotium CF640R Biocytin
Fluorescent biocytin conjugates are cell-impermeant, fixable polar tracers with biotin and an aldehyde-fixable primary amine groups. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R dye has excitation/emission at 642/662 nm.
Biotium CF640R Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R dye has excitation/emission at 642/662 nm.
Biotium CF750, Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent CF750 dye has excitation/emission at 755/777 nm.
Biotium Mix-n-Stain™ CF660C Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF660C dye has excitation/emission at 667/685 nm. It is suitable for labeling ligands for cell surface staining.
Biotium ViaFluor 405 Live Cell Microtubule Stain, Trial Size
ViaFluor™ 405 is a blue fluorescent taxol probe with excitation/emission at 408/452 nm that allows fluorescent staining of microtubules in living cells for detection in the DAPI channel. Supplied at 1000X in DMSO, with a vial of 100 mM verapamil, which can enhance staining in some cell types.
Biotium 5(6)-Tetramethylrhodamine Maleimide
5(6)-Carboxytetramethylrhodamine maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement for 5(6)-carboxytetramethylrhodamine C5-maleimide (91040).Properties: Ex/Em (MeOH) = 540/565 nm; Red solid soluble in DMF or DMSO; Store at -20°C and protect from light, especially in solution; C35H36N4O6; MW: 608.66.