Protein Labeling Reagents
Biotium LysoView™ 405, 1000X in DMSO, Trial Size
LysoView™ 405 is a blue fluorescent lysosome stain for live cells. It has excitation at 318 and 400 nm and emission at 464 nm, for detection in the DAPI or Pacific Blue channels.
Biotium 5-(and-6-)-((N-(5-aminopentyl)amino)carbonyl) Tetramethylrhodamine (Tetramethylrhodamine cadaverine) (mixed isomer)
INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins. Properties: Light yellow solid soluble in DMSO or DMF; Store at 4°C and protect from light; C30H34N4O4; MW: 295.08.
Biotium FAM SE, 5 and 6 isomers
5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate. Properties: Ex / Em = 495/519 nm; Yellow solid soluble in DMSO or DMF; Store at -20°C and protect from light; C 25 H 15 NO 9; MW: 473.4; [117548-22-8]
Biotium CF488A-Dextran 10, 000 MW, Anionic and Fixable
CF dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran conjugates are available in a variety of molecular weights, and contain a formaldehyde-fixable free-amine group. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
Biotium 5-TAMRA-PEO3-amine
5-TAMRA-PEO3-amine (Tetramethylrhodamine-PEO3-amine) has a long water-soluble PEG spacer separating the dye and the amine functional group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides. Properties: Ex/Em (MeOH) = 544/571 nm; Dark purple solid soluble in DMSO, DMF, or H2O; Store at 4°C and protect from light; C 37 H 42 F 6 N 4 O 11; MW: 832.74
STEMCELL Technologies GloCell™ Fixable Viability Dye Violet 540, Size: 500 Tests
GloCell™ Fixable Viability Dye Violet 540 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 540 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 540 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
Biotium CF633 Protein Labeling Kit , 3x(1mg) labelings
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF dye succinimidyl ester (CF dye SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 dye has excitation/emission at 630/650 nm.
Biotium Mix-n-Stain™ CF555 Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange-red fluorescent CF555 dye has excitation/emission at 555/565 nm. It is suitable for labeling ligands for cell surface staining.
Biotium CF647 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF647 dye has excitation/emission at 650/665 nm.
Aat Bioquest iFluor™ 647 amine
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
Aat Bioquest HIS Lite™ Cy3 Bis NTA-Ni Complex
Polyhistidine is one of the most popular affinity tags incorporated into recombinant proteins. It can be inserted either at the N- or C-terminus, and expressed in a variety of hosts.
Biotium Phalloidin, CF488A, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Freen fluorescent CF488A has excitation/emission at 490/515 nm.
Biotium CF633 Maleimide
CF Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 dye has excitation/emission at 630/650 nm.
Biotium Biotin Picolyl Azide
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.