Protein Modification Reagents
MilliporeSigma™ Calbiochem™ Thrombospondin, Human Platelets
Blocks FGF-induced neovascularization of rat cornea.
MilliporeSigma™ Calbiochem TCEP, Neutral
Ready-to-use stable, neutral form of popular disulfide-reducing agent TCEP, Hydrochloride
MP Biomedicals™ Protease, from S. aureus strain V8
Used for selective cleavage of proteins for amino acid sequence determination or peptide mapping
MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction System 1
Induces protein expression without monitoring cell density.
Thermo Scientific™ MS(PEG)24 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.
Thermo Scientific™ Pierce™ Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).
Thermo Scientific™ Pierce™ MMTS (methyl methanethiosulfonate)
Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.
Invitrogen™ Thiol and Sulfide Quantitation Kit
Ultrasensitive colorimetric assay for quantifying both protein and nonprotein thiols
Promega AccuMAP Low pH Protein Digestion Mini Kit, 1 each
AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.
Promega AccuMAP Denaturing Solution, 1ml
AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.
Promega AccuMAP 100X Oxidation Suppressant, 50microl
AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.
Promega ProTEV Plus Protease
ProTEV Plus is an improved 48kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity.
Promega Glycosidases
Endo H is cloned from S. plicatus and overexpressed in E. coli.
Promega AccuMAP Modified Trypsin Solution, 120microl
AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.