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GORE Protein Capture Devices with immobilized Protein A are intended for small-scale affinity purification of monoclonal antibodies and other proteins demonstrating an Fc region. GORE Protein Capture Devices use a unique expanded polytetrafluoroethylene (ePTFE) membrane composite that provides a bindding capacity advantage at high flow rates and improved the speed of purification. In addition the GORE Protein Capture Deivces produce highly concentrated elution pools, which may eliminate a downstream concentration step during the antibody purification process. These unique membrane devices are offered in 1 mL (≥ 30 mg capacity), 3.5 mL (≥ 105 mg capacity) and 9.0 mL (≥27 mg capacity) at 20 seconds residence time.
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GenScript Protein A MagBeads are superparamagnetic beads covalently bound with recombinant protein A for antibody purification. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A MagBeads have a binding capacity of more than 10 mg Rabbit IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).
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Sartorius Octet Protein A Biosensors are designed for rapid and precise quantitation of immunoglobulins, including human IgG, using advanced bioprocessing applications. Their unique Protein A coating allows direct measurement from complex samples, offering speed and accuracy with a throughput of ~32 min for 96 samples. The regeneration feature and high-throughput capabilities make them ideal for critical tasks like protein purification and cell line screening.
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A DNA sequence encoding the Bundibugyo ebolavirus(Subtype Sudan strain Gulu) NP (YP-138520 1) (Gly630-Asp738) was expressed with an N-terminal polyhistidine tagged AVI tag The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed
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