Bioluminescence Assays and Reagents
Amsbio LLC EF1a-Luciferase (firefly)/ (Hygro)
Lucifierase (firefly) expression lentivirus under the enhanced EF1a promoter, with a Hygromycin antibiotic selection under RSV promoter.
Amsbio LLC CMV-Luciferase (firefly)-2A-GFP (Bsd)/ Concentrated Lentivirus
CMV-Luciferase (firefly)-2A-GFP (Bsd) in vivo ready
Amsbio LLC Firefly Luciferase Lentivirus (G418, Hygromycin and Puromycin)-79692-P
Firefly Luciferase Lentivirus Puromycin
BIOHIPPO NFAT-RFP-Luc-Puro LV
NFAT-RFP-Luc-Puro is a transcription factor reporter lentiviral vector designed to monitor NFAT signaling pathway in living cells The construct carries a RFP and luciferase dual reporter under the control of pathway responsive elements and includes a puromycin selection marker to generate stable cell pools The lentivirus is provided as a ready to use VSV G pseudotyped preparation for efficient gene delivery and stable genomic integration Each vial provides a nominal titer of 5x10E6 transducing units TU Typical applications include pathway activation studies assay development compound screening and mechanism of action research The system is suitable for human and mouse cells and can be read using standard fluorescence microscopy flow cytometry or luciferase assays depending on the reporter gene This product is intended for research use only and is not for diagnostic or therapeutic procedures
BIOHIPPO h/m BCL-xL-sh-RFP/Luc
h/m BCL-xL-sh-RFP/Luc shRNA lentiviral vector expressing RFP/Luc with selection for studies in Human/Mouse Each vial provides a nominal titer of 2x10E6 transducing units TU Validated h/m BCL-xL shRNA Lentivirus High-titer shRNA lentiviral particles specific for human/mouse BCL2 like 1 (BCL-xL) The shRNA has been validated to meet or exceed 70% BCL-xL knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene ( 70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs
Cayman Chemical MyrIcetIn 3-galactosIde 5mg
A flavonoid with diverse biological activities; inhibits xanthine oxidase in a cell-free assay at 100 UG/ml; cytotoxic to K562 cells (IC50 = 220 UG/ml); inhibits hydrogen peroxide-induced MDA formation in K562 cells at 110, 220, and 440 UG/ml; reduces carrageenan-induced paw edema in wild-type mice at 0.26 and 0.78 mg/kg; reduces formalin-induced paw licking in mice
BIOHIPPO EF1a-H3.3-K27M-Flag/BSD
EF1a-H3 3-K27M-Flag/BSD lentiviral vector expressing Flag under EF1a promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human H3 3 K27M ORF cDNA lentivirus Lentiviral particles packaged with expression vector for K27M mutant of human H3 3 histone A (also known as H3 3 or H3-3A) transcript variant 1 An EF1 promoter drives expression of H3 3 K27M fused to a C-terminal V5 tag and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP) or luminescent (firefly luc) reporters
Vector Laboratories FIREFLY LUCIFERASE (CMV, BLAST
Firefly Luciferase (CMV, blasticidin) Lentivirus (2x25uL) 2x25uL (1x10^8 CFU/mL); Shipping: Dry ice
ABclonal Technology PEF1 Rabbit mAb
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2 ) signaling Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1