Bioluminescence Assays and Reagents
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Thermo Scientific™ Pierce™ Firefly Signal Enhancer (100X)
Produce maximum bioluminescence signal intensity and sensitivity with the Pierce Firefly Luciferase Flash and Glow Assays.
Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit
Perform firefly luciferase assays in high-throughput screening applications.
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase, Cypridina Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters
| Compatible Cells | Mammalian Cells |
|---|---|
| Substrate Type | Luciferase Substrate |
| Detection Method | Bioluminescence |
| Promoter | EF-1α |
| Substrate | Coelenterazine |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in mammalian cell culture media and whole cell lysate with this highly sensitive flash-based luciferase kit.
| Substrate | Vargulin (Cypridina Luciferin) |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Cypridina Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Invitrogen™ D-Luciferin, Potassium Salt
Needed for luciferin/luciferase light-producing reaction
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ pCMV-Gaussia-Dura Luc Vector for Luciferase Assays
Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.
Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.
| Substrate | Coelenterazine |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Green Renilla Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)
Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.
BD D- Luciferin Potassium Salt
Synthetically made substrate which can be catalyzed by firefly luciferase
| Content And Storage | -20°C |
|---|---|
| Type | D-Luciferin |
| Formulation | Lyophilized |
| For Use With (Application) | Luminometer |
Thermo Scientific™ pEF1α Tluc16-DD Vector for Luciferase Assays
Insert promoter sequences for genes of interest into Thermo Scientific™ Tluc16 Vectors to measure gene regulation using the intracellular TurboLuc™ 16 (Tluc16) luciferase reporter in luciferase assays.
Promega Nano-Glo™ Luciferase Assay System
The Nano-Glo™ Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.
| Content And Storage | −30 to −10°C |
|---|---|
| Type | Nano-Glo Luciferase Assay Buffer |
| For Use With (Application) | Provides a single addition reagent that generates a glow-type signal in the presence of NanoLuc™ luciferase |
Promega Nano-Glo™ Dual-Luciferase™ Reporter Assay System
The Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.
| Content And Storage | -30°C to -10°C |
|---|
Promega pNL3.2[NlucP/minP] Vector
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The Vectors offer a minimal promoter for cloning response elements of interest.
Thermo Scientific™ TurboFect™ Transfection Reagent
Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.
| Type | Transfection Reagent |
|---|---|
| Product Line | TurboFect |