Bioluminescence Assays and Reagents
Revvity Health Sciences Inc twinlite Firefly and Renilla Luciferase Reporter Gene Assay System, 1,000 Assay Points
twinlite Firefly and Renilla Luciferase Reporter Gene Assay System, 1,000 Assay Points
Sigma Aldrich Fine Chemicals Biosciences firefly Luciferase Assay f
firefly luciferase is widely used as a reporter for studying gene regulation and function and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. firefly luciferase is a 62 000 Dalton protein which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. The firefly Luciferase Assay is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working SOLUTIONon to samples. The luminescence signal decays over the course of about 10 minutes of reaction
Cayman Chemical SureLIghtGlow FIrefly Luc
A synthetic peptide substrate can be used as a substrate to quantify calcineurin activity
Cayman Chemical MyrIcetIn 3-galactosIde 10mg
A stimulator of soluble guanylate cyclase and an active metabolite of riociguat increases phosphorylation of the PKG substrate VASP in isolated rat aortic smooth muscle cells (EC50 353 nM) induces vasodilation in rat aortic rings (EC50 75 nM) decreases urine output and cardiac hypertrophy as well as improves survival in spontaneously hypertensive stroke-prone rats fed a high-salt high-fat diet at 3 mg/kg improves erectile responses to electrical stimulation of the cavernous nerve in a rat model of cavernous nerve crush injury at 0 03 and 0 3 mg/kg
Cayman Chemical THUNDRTotal STAT5 TRFRET 9600
An MCT4 inhibitor (IC50 1.3 nM) selectively inhibits lactate influx in NCI H358 cells (IC50 6.1 nM) which highly express MCT4 and do not express MCT1 over lactate influx in K562 cells (IC50 >30 UM) which highly express MCT1 and do not express MCT4 inhibits the proliferation of NCI H358 cells (IC50 26 nM) reduces tumor growth in an NCI H358 mouse xenograft model at 100 mg/kg twice per day in combination with cediranib
Cayman Chemical THUNDRTotal STAT5 TRFRET 4800
An MCT4 inhibitor (IC50 1.3 nM) selectively inhibits lactate influx in NCI H358 cells (IC50 6.1 nM) which highly express MCT4 and do not express MCT1 over lactate influx in K562 cells (IC50 >30 UM) which highly express MCT1 and do not express MCT4 inhibits the proliferation of NCI H358 cells (IC50 26 nM) reduces tumor growth in an NCI H358 mouse xenograft model at 100 mg/kg twice per day in combination with cediranib
Abcam D-Luciferin, chemiluminescent luciferase substrate, 50MG
MW 280.3 Da, Purity >98%. Cell-permeable chemiluminescent luciferase substrate. Emits lights upon oxidative decarboxylation in the presence of ATP. Provides a bioluminescent signal for cellular ATP levels in vivo. Water soluble analogs are D-Luciferin potassium salt (ab143655) and D-Luciferin sodium salt (ab145164).
The product is subject to the following: Abcam Restricted Use Statement
Abcam Recombinant Firefly Luciferase protein, 500UG
Recombinant Firefly Luciferase protein is a Firefly Full Length protein, in the 1 to 550 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Protein/ Peptide/ Enzyme Synonyms: Luciferin 4-monooxygenase, Luciferase
Conjugation: Unconjugated
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Firefly Luciferase mRNA Cap-
A Pim-3 kinase inhibitor (IC50 0.05 UM) selective for Pim-3 over Pim-1 and Pim-2 (IC50s 2.5 UM for both) inhibits the growth of DU145 and MIA PaCa-2 cells (IC50s 0.9 and 3.9 UM respectively) inhibits IL-6-induced STAT3 phosphorylation in DU145 cells at 10 UM
Cayman Chemical CyprIdIna LucIfRIntrIfluor
A natural non-caloric sweetener and hexose monosaccharide metabolized by gut bacteria in the colon of pigs
Cayman Chemical QuercetIn 3-D-galactosIde 1mg
An intermediate in the synthesis of the plant hormone ethylene acts as a partial agonist at the glycine-binding site on NMDA receptors (EC50 0.7-0.9 UM) and in the presence of high levels of glutamate as a competitive antagonist at the glutamate-binding site on NMDA receptors (EC50 81.6 nM) protects against neuron cell death in in vivo models of ischemia
Cayman Chemical QuercetIn 3-D-galactosIde 10mg
An ATP-competitive inhibitor of Cdk1 2 4 5 6 and 9 with IC50 values of 210 47 100 13 170 and 50s 40-940 nM in vitro) and human tumor xenograft models inhibiting cell cycle progression and inducing apoptosis