Bioluminescence Assays and Reagents
Amsbio LLC EF1a-Luciferase (firefly)/ (Hygro)
Lucifierase (firefly) expression lentivirus under the enhanced EF1a promoter, with a Hygromycin antibiotic selection under RSV promoter.
BPS Bioscience Inc STAT3 Reporter Kit (STAT3 Signaling Pathway) | 500 Reactions
The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Cellomics Technology LLC MV4-11/GFP-FIREFLY LUCIFERASE
MV4-11/GFP-firefly luciferase (CMV) stable cells. 2x106 cells
Invivogen J774-Dual Cells, 3-7 x 10e6 cells
J774-Dual cells have been derived from the mouse J774.1 macrophage-like cell line by stable integration of two inducible reporter constructs.
Invivogen A549-Dual Cells, 3-7 x 10e6 cells
A549-Dual cells are adherent epithelial cells that have been derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs. The A549 cell line is a well-characterized cellular model for asthma, allergies and respiratory infections.
Invivogen HCT116-Dual Cells, 3-7 x 10e6 cells
HCT116-Dual cells are adherent epithelial cells that have been derived from the human HCT116 colorectal carcinoma cell line by stable integration of two inducible reporter constructs. HCT116-Dual™ cells express numerous pattern recognition receptors (PRRs), including the NOD-like receptors (NLRs) NOD1 and NOD2, the RIG-I-like receptor (RLR) RIG-I, and the Toll-like receptors (TLRs) TLR3 and TLR5 but not TLR2 or TLR4.
BPS Bioscience Inc RENILLA LUCIFERASE LENTIVIRUS
500ulx2 The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS