General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR37 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR37 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR37 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx AssayComplete Cell Plating 19 Reagent, 500. (93-0563R19B)
AssayComplete Cell Plating 19 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress TrkC-P75 Functional Assay, 2-Plate Kit. (93-0531E3CP16M)
The PathHunter eXpress TrkC-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices FLIPR Calcium Assay Kit, Express, Fluorescent, 96/384/1536-Plate
The FLIPR Calcium 6 Express Kit is a fluorescence-based, homogeneous reagent set for detecting intracellular calcium flux in high-throughput screening. The kit delivers an enhanced signal window and improved resistance to organic anion transporters, reducing the need for probenecid and enabling robust GPCR and ion channel assays across 96-, 384-, and 1536-well formats.
- Enhanced signal window for improved assay sensitivity.
- Homogeneous fluorescence format for simplified workflows.
- Reduced need for probenecid due to dye resistance to organic anion transporters.
- Supplies sufficient reagent for approximately 100 96- or 384-well plates, or 150 1536-well plates.
- Store reagents at -20°C; aliquots may be frozen for short-term use.
Eurofins Discoverx PathHunter Bioassay Kit ED Detection Kit, 10 (200 dp, 96-well). (93-1043E)
The PathHunter Bioassay ED Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 2 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-1043e.
TRIBIOSCIENCE INC Homocysteine Assay (Colorimetric / Fluorometric) 100 Tests
Tribioscience’s Homocysteine Assay measures homocysteine concentrations in plasma or serum and other bio-samples with coupling enzyme reactions. The resulting fluorescent signal at Ex/Em = 400/465 nm is in proportion to homocysteine concentrations in samples. The kit provides the easiest and most accurate approach to measuring homocysteine levels in a sample. Applications - Measure Homocysteine in a variety of samples.
Abcam Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Assay Kit.
Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress HRH2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0208E2CP0L)
The PathHunter HRH2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH2 and EA-tagged β-Arrestin-2. Activation of HRH2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress Flt4 Activity Assay, 10-Plate Kit. (93-0888E3CP16L)
The PathHunter FLT4 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activity via detection of resulting FLT4 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FLT4 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0192E2CP0M)
The PathHunter CCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL2/MCP-1) based activation of CCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR2 and EA-tagged β-Arrestin-2. Activation of CCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GPR35 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0152E2CP2L)
The cAMP Hunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR35, a naturally Gi/Go coupled GPCR. Upon activation of GPR35, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Culture Kit-117, (92-3117G)
AssayComplete Cell Culture Kit-117 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Revvity Health Sciences Inc AlphaPlex 545 (Terbium) Human ICAM-1 Detection Kit, 500 Assay Points
AlphaPlex 545 (Terbium) Human ICAM-1 Detection Kit, 500 Assay Points
Indigo Biosciences Human Adrenoceptor Alpha 1A (ADRA1A) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 384-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1A reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1A. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences Human Adrenoceptor Alpha 1A (ADRA1A) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1A reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1A. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.