Cell Based Assays
Cell Based Assays
Members of the RAS superfamily of GTP-binding proteins are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions
Histone Deacetylase Assay (HDAC)
Immunocytochemistry, Transfection, Immunofluorescence
Measures glutathione peroxidase (GPx) activity in plasma, erythrocyte lysates, tissue homogenates and cell lysates
Single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
ChIP analysis at nucleosome resolution by performing complete or partial digestions with a proprietary enzymatic cocktail to obtain chromatin fragments of on average one to a few nucleosomes in length
For reproducible multicolor phenotyping of MSC expansions
Purified from pooled rat plasma.
Provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD
Immunofluorescence, Flow Cytometry, Activity Assay
|For Use With (Application)||Immunofluorescence, Flow Cytometry, Activity Assay|
For detection of active caspase-8 in cells undergoing apoptosis
Ready-to-use solution of the nucleic acid dye, 7-Amino-actinomycin D (7-AAD) that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays
|Content And Storage||4°C|
Formulated for use in flow cytometric assays utilizing Annexin V
For research use only
Cells with active caspases can be detected. BD Pharmingen™ Blue Live Cell Caspase Probe contains a fluorochrome-labeled caspase inhibitor. Fluorochrome-labeled caspase inhibitors have three functional domains: fluorochrome , 3-amino acid sequence (VAD) that binds to the active site of the activated caspase, and fluoromethyl ketone (FMK) moiety that allows the probe to irreversibly bind the active caspase.