General Cell Based Assays
Meso Scale Discovery U-PLEX Human TNF-B ASY 25 PL
The Meso Scale Discovery U-PLEX Human TNF-beta Assay Kit is a multiplex immunoassay that measures human TNF-beta in serum plasma cell culture supernatants and other biological samples It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay can be run on Meso Scale Discovery instrumentation including the QuickPlex SQ 120 QuickPlex SQ 120i MESO QuickPlex SQ 120 and V-PLEX Plus The kit includes a 96-well plate coated with capture antibodies detection antibodies calibrators and ancillary reagents needed to perform the assay
Molecular Devices Fura-2 QBT Calcium Kit, Explorer, 10-Plate, 96/384/1536-Well
The Fura-2 QBT Calcium Explorer Kit from Molecular Devices is a homogeneous, ratiometric assay kit for plate-based calcium measurements. The kit uses a quench-based format with a Fura-2 indicator to enable no-wash assays and reduce cell-based variability.
- Homogeneous ratiometric calcium assay that eliminates wash steps.
- Explorer format contains reagents sufficient for ten microplates.
- Each vial of Component A is sufficient for one microplate.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Uses a ratiometric Fura-2 indicator with ultraviolet excitation to reduce fluorescent interference.
Eurofins Discoverx PathHunter eXpress TrkC-P75 Functional Assay, 10-Plate Kit. (93-0531E3CP16L)
The PathHunter eXpress TrkC-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR109B CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0791E2CP2L)
The PathHunter GPR109B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 3-hydroxyoctanoic acid) based activation of GPR109B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109B and EA-tagged β-Arrestin-2. Activation of GPR109B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mADCYAP1R1 (PAC1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0594E2MCP0L)
The PathHunter mADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of mADCYAP1R1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mADCYAP1R1 and EA-tagged β-Arrestin-2. Activation of mADCYAP1R1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress RXFP3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0692E2CP2L)
The PathHunter RXFP3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged RXFP3 and EA-tagged β-Arrestin-2. Activation of RXFP3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR142 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0413E2ACP1L)
The PathHunter GPR142 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR142 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR142 and EA-tagged β-Arrestin-2. Activation of GPR142 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cellular Dynamics International iCell Microglia Media Kit, 50 ml iCell Glial Base Medium, 0.5 ml iCell Microglia Supplement A (100x), 0.5 ml iCell Microglia Supplement B (100x), 1 ml iCell Neural Supplement C (50X)
The iCell Microglia Media Kit is a specialized cell culture medium tailored for the optimal growth and maintenance of human iPSC-derived microglia. It provides a consistent in vitro environment, essential for research in microglial biology and neurodegenerative diseases. This media kit solves the challenges faced with primary human microglia by ensuring reproducible results for various experiments related to neuroinflammation and drug discovery.
- Supports growth of iPSC-derived human microglia.
- Includes multiple supplements for enhanced performance.
- Stores complete medium at 4°C for up to 2 weeks.
- Can be aliquoted for single-use storage at -20°C for up to 3 months.
- Recommended culture conditions: 37°C and 5% CO2.
- Designed for research purposes only, focusing on neurodegeneration and drug discovery.
- Maintains relevant microglial markers and physiological functions.
Eurofins Discoverx PathHunter Human BDKRB2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human BDKRB2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure BDKRB2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx SPRINTer™ Human BRD4 Targeted Protein Degradation Cell-Based Assay Kit (HCT-116)
The SPRINTer™ Human BRD4 Targeted Protein Degradation Stable Cell Line Assay (HCT-116) contains a stable clonal cell line used for monitoring drug-induced protein turnover of BRD4, supporting TPD studies. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR139 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR139 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR139 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR152 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human GPR152 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure GPR152 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx HitHunter cGMP Assay, 10,000 dp (384-well). (90-0039L)
The HitHunter cGMP Assay is a detection kit offering a robust, sensitive and easy-to-use assay to measure intracellular cGMP levels. This product uses EFC technology, and contains ED-tagged cGMP, anti-cGMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cGMP, the anti-cGMP antibody will be sequestered by binding to this untagged cGMP, allowing for EFC between the tagged cGMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit may be used with Eurofins DiscoverX cell lines as well as cell lines from other sources. This detection kit contains reagents for 25 plates (384 well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter eXpress NTSR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0671E3CP5M)
The PathHunter NTSR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NTSR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged NTSR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting NTSR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR7 Activated GPCR Internalization Assay, 10pl Kit. (93-0738E3CP27L)
The PathHunter CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR7 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.