General Cell Based Assays
Bioassay Systems EnzyChrom™ Creatine Assay Kit. For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.
For quantitative determination of creatine and evaluation of drug effects on creatine metabolism. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Linear detection range 4 to 1000 µM (colorimetric) or 0.5 to 50 µM (fluorimetric). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes. Method: OD570nm, or FL530/590nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 4 µM.
Bioassay Systems QuantiChrom™ Creatinine Assay Kit. For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism.
For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).
Bioassay Systems QuantiFluo™ Ammonia Assay Kit. For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm (compatible on handheld fluorimeter catalog# FL360450).
For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological (eg urine) and environ-mental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 200 tests. Detection limit: 12 µM.
Bioassay Systems QuantiChrom™ Calcium Assay Kit. For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism.
For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca2+ in 96-well plate assay. Simple and high-throughput. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).
AnaSpec SensoLyte Green Glutaminyl Cyclase Activity Assay *Fluorimetric*, 1 Kit
SensoLyte Green Glutaminyl Cyclase Activity Assay *Fluorimetric*, 1 Kit, The SensoLyte Green Glutaminyl Cyclase Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring enzyme activity from various sources using a green fluorescence substrate. During the first step, substrate is incubated with Glutaminyl Cyclase or enzyme-containing samples and converted into the pyroglutamate form. Secondly, glutaminyl cyclase developer is added to remove pGlu residue and generate the green fluorophore. It can be detected with excitation at 490 nm and emission at 520 nm. Produced fluorescence is proportional to the enzyme activity. This kit is ideal for high throughput screening (HTS) of glutaminyl cyclase activators and inhibitors. The long wavelength of the green fluorophore is less interfered by autofluorescence of cell components and test compounds. Assay sensitivity is 1.5 ng/mL of active enzyme.
Bioassay Systems EnzyChrom™ Ascorbic Acid Assay Kit. For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.
For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, and cell culture. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: OD, FL: 6, 1 µM.
Bioassay Systems QuantiChrom™ Uric Acid Assay Kit. For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism.
For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 mg/dL (13 µM).
Bioassay Systems EnzyLight™ ATP Assay Kit. For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.
For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ATP or a single cell can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM.
Bioassay Systems QuantiChrom™ Antioxidant Assay Kit. For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism.
For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range from 1.5 to 1000 µM Trolox equivalents. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD570nm. Samples: Serum, plasma, urine, saliva, food and beverage etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1.5 µM.
Bioassay Systems PiBlue™ Phosphate Assay Kit. For sensitive and high-throughput phosphate determination.
For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure". Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.
Bioassay Systems EnzyChrom™ Oxaloacetate Assay Kit. For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism.
For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Linear detection range: 7 to 400 µM oxaloacetate for colorimetric assays and 1 to 40 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue and culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 1 µM.
Bioassay Systems EnzyChrom™ Superoxide Dismutase Assay Kit. For quantitative determination of superoxide dismutase (SOD) enzyme activity.
For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects. Key Features: Sensitive and accurate. Linear detection range of 0.05 - 3 U/mL SOD. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD440nm. Samples: Blood, cell, tissue etc. Species: All. Procedure: Assay takes 1 hr. Kit size: 100 tests. Detection limit: 0.05 U/mL.
Bioassay Systems EnzyChrom™ Sialic Acid Assay Kit. For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µM for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 µM.
Bioassay Systems EnzyChrom™ Pyruvate Assay Kit. For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µM.