General Cell Based Assays
Eurofins Discoverx PathHunter eXpress OXTR Activated GPCR Internalization Assay, 10pl Kit. (93-0715E3CP0L)
The PathHunter OXTR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OXTR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OXTR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OXTR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ErbB4/ErbB4 Dimerization Assay, 2pl Kit. (93-0961E3CP5M)
The PathHunter ErbB4/ErbB4 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. neuregulin-1 Beta 2) induced dimerization of ErbB4/ErbB4 receptors. This product uses EFC technology. The cell line included overexpresses ErbB4 target protein fused to PK alongside ErbB4 target protein fused to EA. Upon ligand-induced dimerization of ErbB4/ErbB4, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2 Dimerization Assay, 10pl Kit. (93-0963E3CP0L)
The PathHunter ACVRL1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR2 target protein fused to PK alongside ACVRL1 target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rVIPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0913E2RCP2M)
The PathHunter rVIPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of rVIPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rVIPR1 and EA-tagged β-Arrestin-2. Activation of rVIPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress BMPR1A/BMPR2 Dimerization Assay, 2pl Kit. (93-1006E3CP0M)
The PathHunter BMPR1A/BMPR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. BMP-4) induced dimerization of BMPR1A/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses BMPR2 target protein fused to PK alongside BMPR1A target protein fused to EA. Upon ligand-induced dimerization of BMPR1A/BMPR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mFPR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-1034E2MCP2L)
The PathHunter mFPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. formylpeptides) based activation of mFPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mFPR2 and EA-tagged β-Arrestin-2. Activation of mFPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TGFBR1/TGFBR2 Dimerization Assay, 10-plate Kit. (93-0889E3CP5L)
The PathHunter TGFBR1/TGFBR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TGF-β1) induced dimerization of TGFBR1/TGFBR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside TGFBR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/TGFBR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR1 Total GPCR Internalization Assay, 2pl Kit. (93-0729E3CP0M)
The PathHunter HCRTR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY4 Activated GPCR Internalization Assay, 2pl Kit. (93-0741E3CP0M)
The PathHunter P2RY4 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY4 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY4. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY4-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 Activated GPCR Internalization Assay, 10pl Kit. (93-0655E3CP5L)
The PathHunter CHRM1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGIR Total Internalization Assay, 2pl Kit. (93-0838E3CP0M)
The PathHunter PTGIR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGIR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TORC1 Nuclear Translocation Assay, 10pl Kit. (93-0833E3CP19L)
The PathHunter eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc Glutamate Assay Kit (Fluorometric), 200 assays
Excess glutamate levels in the brain can cause cell injury and death, leading to neurological diseases. Cell Biolabs' Glutamate Assay Kit is a quantitative, fluorometric assay that uses glutamate specific enzymes to generate hydrogen peroxide. Glutamate levels as low as 300 nM can be detected in serum, plasma, urine, cell lysates, and cell culture supernatants. Each kit provides sufficient reagents for 200 assays including standards and unknown samples.
Eurofins Discoverx cAMP Hunter eXpress GIPR CHO-K1 GPCR Assay, 10-Plate Kit. (95-0146E2CP2L)
The cAMP Hunter GIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GIPR, a naturally Gs coupled GPCR. Upon activation of GIPR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.