General Cell Based Assays
Eurofins Discoverx PathHunter eXpress AGTRL1 Activated GPCR Internalization Assay, 10pl Kit. (93-0710E3CP0L)
The PathHunter AGTRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AGTRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress c-KIT Functional Assay, 10-Plate Kit. (93-0853E3CP16L)
The PathHunter eXpress c-KIT Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-KIT dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged c-KIT and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the cynomolgus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL17RA/IL17RC Dimerization Assay, 10-plate Kit. (93-0931E1CP7L)
The PathHunter IL17RA/IL17RC Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-17a) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL5RA/CSF2RB Dimerization Assay, 10pl Kit. (93-0972E3CP0L)
The PathHunter IL5RA/CSF2RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-5) induced dimerization of IL5RA/CSF2RB receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside IL5RA target protein fused to EA. Upon ligand-induced dimerization of IL5RA/CSF2RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical 23-cGAMPsodIum salt 500ug
A second messenger produced from ATP and GTP by cGMP-AMP synthase (cGAS) in the cytoplasm of mammalian cells in response to the presence of DNA; contains a non-canonical 2’5’-phosphodiester bond; binds tightly to the adapter protein STING (Kd = 3.79 nM) and induces IFN-β mRNA expression (EC50 = 19.4 nM in L929 cells)
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 2pl Kit. (93-0968E3CP5M)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical MItoLIteOrnge FX570 500test
A mitochondrial dye for live and fixed cells; ex/em = 545/575 nm; has been used to label mitochondria in Hep2 cells treated with cisplatin and NAC
Eurofins Discoverx PathHunter eXpress UTR2 Total GPCR Internalization Assay, 10-Plate Kit. (93-0746E3CP5L)
The PathHunter UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR7 Activated GPCR Internalization Assay, 10pl Kit. (93-0738E3CP27L)
The PathHunter CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR7 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices cGMP Fluorescent Assay Kit, 96-Well, Bulk, Competitive Immunoassay
CatchPoint cGMP Fluorescent Assay Bulk Kit provides a high-sensitivity, 96-well competitive immunoassay for quantitative measurement of cGMP and guanylate cyclase activity. The bulk format supplies reagents and ten pre-coated plates for high-throughput biochemical and cell-based assays.
- High-affinity reagents optimized for low cGMP levels.
- Ten pre-coated black clear-bottom 96-well plates in bulk format.
- Sufficient reagents for ten 96-well plates (960 reactions at 120 μL per well).
- Competitive immunoassay with a single washing step.
- Flexible read window: 10 minutes to 24 hours after substrate addition.
- Store components at 4°C; unopened kit stable for six months.
Molecular Devices Fura-2 QBT Calcium Kit, Explorer, 10-Plate, 96/384/1536-Well
The Fura-2 QBT Calcium Explorer Kit from Molecular Devices is a homogeneous, ratiometric assay kit for plate-based calcium measurements. The kit uses a quench-based format with a Fura-2 indicator to enable no-wash assays and reduce cell-based variability.
- Homogeneous ratiometric calcium assay that eliminates wash steps.
- Explorer format contains reagents sufficient for ten microplates.
- Each vial of Component A is sufficient for one microplate.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Uses a ratiometric Fura-2 indicator with ultraviolet excitation to reduce fluorescent interference.
Molecular Devices Calcium 6 Assay Kit, Bulk, 100 Plates, 96/384/1536-Well
The FLIPR Calcium 6 Bulk Kit is a reagent set formulated for high-throughput calcium flux assays, supplied in a bulk format to support large screening campaigns. The kit provides multiple vials of the primary dye component and is compatible with common microplate formats and FLIPR/FlexStation readers.
- Bulk format for 100 plates.
- Includes 10 vials of Component A; each vial sufficient for multiple plates.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Assay buffer (Component B) not included; requires separate preparation or purchase.
- Optimized for calcium flux assays on plate readers and high-throughput screening systems.
Molecular Devices Membrane Potential Assay Kit, Red, Explorer, Homogeneous Fluorescence
The FLIPR Membrane Potential Assay Kit (Red, Explorer) is a homogeneous, fluorescence-based reagent kit for sensitive detection of rapid changes in cellular membrane potential. It is optimized for high-throughput screening and fast kinetic measurements and is compatible with automated plate readers and FLIPR/Tetra systems.
- Homogeneous fluorescence formulation for rapid membrane potential detection.
- Red indicator optimized for larger signal window and temperature stability.
- Explorer format supplies reagents for approximately 10-15 microplates.
- Compatible with 96-, 384-, and 1536-well plate formats.
- Suitable for ion channel and transporter assays, including TRP and voltage-gated channels.
- Free of animal-derived components.
Molecular Devices cAMP Fluorescent Assay Kit, 384-Well, Bulk, 60 μL
The CatchPoint cAMP Fluorescent Assay Kit is a high-throughput competitive immunoassay for quantifying 3',5'-cyclic adenosine monophosphate (cAMP) and measuring adenylate cyclase activity in biochemical and cell-based assays. The 384-well bulk format supplies reagents for 20 plates (7,680 assay points) and is optimized for fluorescence intensity detection with long signal stability.
- 384-well bulk format, sufficient for 20 plates (7,680 wells)
- Reaction volume 60 μL per well, 50 μL supported for some HTS systems
- Fluorescence intensity detection using Stoplight Red substrate
- Minimum detectable concentration 0.1 nM (6 fmol/well), EC50 approximately 3.4 nM
- Readings stable from 10 minutes up to 24 hours after substrate addition
- Store components at 4°C; kit components stable for six months when stored properly