General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress GRM2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0148E2CP23L)
The cAMP Hunter GRM2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. L-glutamate) based activation of GRM2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GRM2, a naturally Gi/Go coupled GPCR. Upon activation of GRM2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Glucose6Phosphate Fluoromet 96
Glucose-6-phosphate (G6P) plays a key role in the homeostatic regulation of blood glucose levels by participating in glycolysis, glycogen synthesis, gluconeogenesis, and can be metabolized to NADPH, enabling protection against oxidative damage. Disruption of G6P activity leads to glycogen storage disease type I or von Gierke’s disease, a group of inherited metabolic diseases characterized by severe hypoglycemia, growth retardation, and hepatomegaly, due to accumulation of glycogen and fat in the liver. Cayman’s Glucose-6-Phosphate Fluorometric Assay provides a fluorescence-based method for detecting G6P in tissue homogenates and cell culture samples. In the assay, G6PDH catalyzes the oxidation of G6P to 6-phospho-D-gluconate, along with the concomitant reduction of NADP+ to NADPH. NADPH reacts with the fluorometric detector to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Revvity Health Sciences Inc HTRF Human & Mouse Total AMPK Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total AMPK Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Tau Aggregation Kit, 500 Assay Points
HTRF Tau Aggregation Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress mOPRM1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0524E3MCP5L)
The PathHunter mOPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of mOPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRM1 and EA-tagged β-Arrestin-2. Activation of mOPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TRIBIOSCIENCE INC Lactic Lactate Fluorometric Assay 100 Tests
Tribioscience’s Lactate Fluorometric Assay Kit is a simple, sensitive, and fast assay that measures the total amount of L-Lactate in biological samples. Lactate is oxidized by lactate oxidase to pyruvate and hydrogen peroxide. Horseradish peroxidase (HRP) catalyzes the reaction between hydrogen peroxide and the specific probe to yield a final product measured by the fluorometric method at Ex/Em=530/590 nm.
Eurofins Discoverx PathHunter Human IL-7 (IL7R/IL2RG) Dimerization Cell-Based Assay Kit (DLD1)
The PathHunter Human IL-7 (IL7R/IL2RG) Stable Cell Line Dimerization Assay (DLD1) measures ligand-induced dimerization of IL7R/IL2RG. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cell Biolabs Inc OxiSelect™ Comet Assay Kit (3-Well Slides), 75 assays (25 slides)
The comet assay, or single cell gel electrophoresis assay (SCGE), is a common technique for measurement of DNA damage in individual cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic “comet tail” shape under the microscope. The extent of DNA damage is usually visually estimated by comet tail measurement; however, image analysis software is also available for measuring various parameters. The OxiSelect™ Comet Assay is a fast and sensitive kit for the measurement of cellular DNA damage. Each kit provides sufficient reagents to perform up to 25 assays (25 slides with 3 wells each).
Revvity Health Sciences Inc AlphaScreen FITC Detection Kit, 50,000 Assay Points
AlphaScreen FITC Detection Kit, 50,000 Assay Points
Cayman Chemical MItochondrl Complx V NADH 3mg
A GC-A positive allosteric modulator induces the production of cGMP in the presence (EC50 0.45 UM) but not the absence of ANP in HEK293 cells expressing human GC-A selective for GC-A over GC-B in HEK293 cells expressing the human receptors at 10 UM binds to the GC-A extracellular domain (Kd 397 nM) and enhances the binding of ANP to the GC-A extracellular domain in cell-free assays enhances ANP-induced production of cGMP in primary human renal proximal tubular cells visceral adipocytes and cardiomyocytes from 1-10 UM inhibits TGF-ß1-induced hypertrophy of primary human cardiomyocytes in combination with ANP induces cGMP production in GC-A-expressing HEK293 cells incubated with plasma isolated from patients with hypertension or heart failure
Eurofins Discoverx PathHunter Human SSTR3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human SSTR3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure SSTR3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Rhod-4 Calcium Assay kit - Medium Removal No Wash.
Rhod-4 Calcium Assay kit - Medium Removal No Wash.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Pyruvate Kinase Assay Kit
Pyruvate Kinase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement