General Cell Based Assays
Eurofins Discoverx PathHunter eXpress MRGPRX2 CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0309E2CP0L)
The PathHunter MRGPRX2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. coristatin-14) based activation of MRGPRX2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX2 and EA-tagged β-Arrestin-2. Activation of MRGPRX2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter C5AR1 Bioassay Kit, 2-Plate. (93-0557Y2-00059)
The PathHunter C5AR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Lipolysis Assay Kit (Colorimetric)
Lipolysis Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Sialic Acid (NANA) Assay Kit
Sialic Acid (NANA) Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Cell Biolabs Inc OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit, 200 assays
Cell Biolabs’ OxiSelect™ Total Antioxidant Capacity (TAC) Assay Kit measures the total antioxidant capacity of biomolecules from a variety of samples via a SET mechanism. The TAC Assay is based on the reduction of copper (II) to copper (I) by antioxidants such as uric acid. Upon reduction, the copper (I) ion further reacts with a coupling chromogenic reagent that produces a color with a maximum absorbance at 490 nm. Copper is advantageous over iron-based antioxidant assays because all classes of antioxidants, including thiols, are detected with marginal radical interference. Cell Biolabs’ OxiSelect™ TAC Assay Kit is a fast and reliable kit for the direct measurement of total antioxidant capacity from cell lysate, plasma, serum, urine, tissue homogenates, and food extracts. Each kit provides sufficient reagents to perform up to 200 assays, including blanks, antioxidant standards and unknown samples. Both hydrophilic and lipophilic samples are compatible with the assay.
Sigma Aldrich Fine Chemicals Biosciences Proteasome 20S Activity As
Protein degradation occurs most commonly through two major mechanisms lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis DNA repair endocytosis and cell cycle and has been implicated in disease states such as certain types of cancer diabetes mellitus and Alzheimer disease among others making it the target f
Abcam N epsilon (Carboxyethyl) Lysine (CEL) Assay Kit
N epsilon (Carboxyethyl) Lysine (CEL) Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Apexbio Technology LLC DiscoveryProbe™ PI3K/Akt/mTOR Compound Library 100uL/well(10 mM solution),96 Well Deep Well Plate
The DiscoveryProbe PI3K/Akt/mTOR Compound Library comprises 178 small molecule inhibitors targeting PI3K/Akt/mTOR-related signaling pathways This pathway regulates processes such as cell growth proliferation survival apoptosis and metabolism in various cell types making it a frequent focus for oncology and disease mechanism research The collection encompasses compounds approved by regulatory agencies including FDA and EMA and agents studied extensively in academic and pharmaceutical research settings Representative inhibitors within this library include PI3K-selective inhibitors ATM/ATR inhibitors (DNA damage response regulators) and DNA-PK and GSK-3 targeting compounds providing comprehensive coverage for biological pathway elucidation drug discovery screening and pharmacological mechanism-of-action studies
Abcam Glycogen Assay Kit II (Colorimetric)
Glycogen Assay Kit II (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Molecular Devices Membrane Potential Assay Kit, Evaluation, 96-Well
The FLIPR Membrane Potential Assay Kit (Evaluation) is a homogeneous, fluorescence-based reagent kit for detecting real-time changes in cellular membrane potential in microplate assays. It provides blue and red dye formulations and reagents sufficient for multiple 96-well assays.
- Homogeneous fluorescence membrane potential assay for real-time measurements.
- Includes blue and red component A dye vials and a buffer bottle.
- Sufficient reagent for 10 microplates (96- or 384-well).
- Designed for use with fluorescence plate readers and screening systems.
- Suitable for ion channel screening and membrane potential functional assays.
- Mix-and-read, no-wash protocol for high-throughput workflows.
Abcam Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam D 2 Hydroxyglutarate Assay Kit (Colorimetric)
D 2 Hydroxyglutarate Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Collagen Degradation/Zymography Assay Kit (Fluorometric)
Collagen Degradation/Zymography Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam SRB Assay / Sulforhodamine B Assay Kit
SRB Assay / Sulforhodamine B Assay Kit
The product is subject to the following: Abcam Restricted Use Statement