General Cell Based Assays
Meso Scale Discovery R-PLEX Human Neprilysin ASY
The R-Plex Human Neprilysin Assay from Meso Scale Discovery is a 96-well plate assay using electrochemiluminescence (ECL) for quantifying human neprilysin in biological samples It features a pre-coated plate with an ECL label-conjugated antibody specific for neprilysin Samples bind to the immobilized antibody and a ruthenylated detection antibody is added ECL is induced by a read buffer and measured with the MSD instrument with the signal proportional to neprilysin levels The assay offers high sensitivity a wide dynamic range and results in 90 minutes
Eurofins Discoverx AssayComplete Cell Culture Kit-115, (92-3115G)
AssayComplete Cell Culture Kit-115 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
CELL SIGNALING TECHNOLOGY INC GALACTOSIDASE ACTIVITY ASSAY K
NC2601719 GALACTOSIDASE ACTIVITY ASSAY K
Abcam Creatinine Assay Kit
Creatinine Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Adipogenesis Assay Kit
Cayman’s Adipogenesis Assay provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model, using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis, and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.
Cayman Chemical Glycerol Colorimetric Assay K
Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various conditions. The measurement of glycerol is also useful for correction of glycerol interference when measuring triglycerides in reference materials and in serum from patients with elevated glycerol concentrations. Cayman’s Glycerol Assay provides a simple, reproducible, and sensitive tool for measurement of glycerol in plasma and serum. The assay employs a coupled enzymatic reaction system that yields a brilliant purple product with an absorbance maximum at 540 nm.
Cayman Chemical Phosphatidylcholine Colorimet
Cayman’s PC Assay provides a specific, sensitive, and convenient method for quantifying PC in plasma or serum. In this assay, PC-Specific PLD hydrolyzes PC to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue dye with an optimal absorption at 595 nm.
Cayman Chemical EcolI PhagocytosIs asy KI
Cayman’s E. coli Phagocytosis Assay Kit employs FITC-labeled, heat-inactivated E. coli cells for the measurement of the phagocytic process in vitro. The engulfed fluorescent E. coli can be detected by flow cytometry.
Cayman Chemical Total nd DIrect BIlIrubIn Co
Cayman’s Total and Direct Bilirubin Colorimetric Assay provides a rapid, reliable, and reproducible colorimetric method for measuring total and direct bilirubin levels in serum. This kit uses a modified Jendrassik-Gróf method, in which total bilirubin concentration is determined by diazo salt in the presence of a catalyst and direct bilirubin is determined in the absence of the catalyst.{55801,55802} The Jendrassik-Gróf method is a widely used clinical method.{55806} However, the results may not be comparable to the results of analytical methods such as mass spectrometry or NMR, and this kit is designed for research use only (not to be used for clinical purposes). The assay has a dynamic range of 0.045-8 mg/dl (0.022-4 μg/well) for both total and direct bilirubin.
Cayman Chemical UrIc AcId asy KIt 96Wel
Cayman’s Uric Acid Assay Kit provides fluorescence- or colorimetric-based methods for detecting uric acid in plasma, serum, and urine. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide (H2O2), and carbon dioxide. H2O2, in the presence of horseradish peroxidase, reacts stoichiometrically with ADHP (10-acetyl-3,7-dihydroxyphenoxazine) to produce the highly fluorescent compound resorufin. Resorufin fluorescence can be analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.
Amsbio LLC Sulfated Glycosaminoglycan Quantification Kit (Proteoglycan Detection)
Sulfated Glycosaminoglycan Quantification Kit (Proteoglycan Detection)
STEMCELL Technologies Intracellular Permeabilization, 150 mL
Reagent for the permeabilization of fixed cells
Proteintech Group Inc Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-1 Method)
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-I method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.