General Cell Based Assays
Eurofins Discoverx PathHunter Human HTR2C (5-HT2C) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human HTR2C(VNV) (5-HT2C) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure HTR2C(VNV) (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter CXCR5 Bioassay Kit, 2-Plate. (93-0514Y2-00063)
The PathHunter CXCR5 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CXCL13) based activation of CXCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR5 and EA-tagged β-Arrestin-2. Activation of CXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter eXpress PTGER2 DLD1 GPCR Assay, 2-Plate Kit. (95-0176E13CP25M)
The cAMP Hunter PTGER2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTGER2, a naturally Gs coupled GPCR. Upon activation of PTGER2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress PTGER2 DLD1 GPCR Assay, 10-Plate Kit. (95-0176E13CP25L)
The cAMP Hunter PTGER2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTGER2, a naturally Gs coupled GPCR. Upon activation of PTGER2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Sargramostim Bioassay Kit, 2-Plate. (93-1078Y3-00111)
The PathHunter Sargramostim Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses GM-CSF or sargramostim-induced dimerization of CSF2RB and CSF2RA receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside CSF2RA target protein fused to EA. Upon ligand-induced dimerization of CSF2RB and CSF2RA, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical CelBased asy CyclohexImId 50uL
A peptide MC1R antagonist (IC50 17 nM) selectively binds to MC1R over MC3R and MC5R (IC50s 3900 and 1000 nM respectively) as well as MC4R at 10 UM increases the latency to tail withdrawal in the tail-flick test in female but not male mice in a model of opioid-induced hyperalgesia at 7.5 UG/animal i.c.v. reduces protein levels of merlin in the cortex reduces the number of surviving neurons in the cortex and hippocampal CA2 region and increases the number of apoptotic cells in the cortex in rats 24 hours after injury in a model of controlled cortical impact-induced TBI at 1 nmol/animal i.c.v.
