General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CSF2RB/CSF2RA Dimerization Assay, 2pl Kit. (93-1078E3CP22M)
The PathHunter CSF2RB/CSF2RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GM-CSF) induced dimerization of CSF2RB/CSF2RA receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside CSF2RA target protein fused to EA. Upon ligand-induced dimerization of CSF2RB/CSF2RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PROKR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0282E2CP2L)
The PathHunter PROKR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PROKR2 and EA-tagged β-Arrestin-2. Activation of PROKR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR92 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0372E2CP6L)
The PathHunter GPR92 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of GPR92 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR92 and EA-tagged β-Arrestin-2. Activation of GPR92 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress HRH3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0103E2CP2L)
The cAMP Hunter HRH3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HRH3, a naturally Gi/Go coupled GPCR. Upon activation of HRH3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter PTH Bioassay Kit, 10-Plate. (93-0315Y2-00048)
The PathHunter PTH Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses PTH based activation of PTHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR and EA-tagged β-Arrestin-2. Activation of PTHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress PTHR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0315E2CP0M)
The PathHunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR1 and EA-tagged β-Arrestin-2. Activation of PTHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
IPHASE PHARMA SERVICES LLC 5α-Reductase(SRD5A1) Inhibition Kit,200 test (Micropore)
Mainly used in 5α-Reductase(SRD5A2) Inhibition study. It's recommended to purchase the complete reagent kit.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0742
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0742
Cayman Chemical NFAT InhIbItr 1mg
A peptide inhibitor of NFAT; binds to the NFAT recognition site on calcineurin (IC50 = 25 pM) but does not inhibit calcineurin phosphatase activity at 100 µM; inhibits the dephosphorylation of NFAT in HEK293T lysates expressing NFAT1, -2, or -4; inhibits NFAT activation in RAW 264.7 macrophages, EA.hy926 endothelial cells, and isolated mouse thoracic aorta VSMCs (IC50s = 29.1, 30.2, and 45.7 µM, respectively, in reporter assays)
Cayman Chemical DIhydroethIdIum 10mg
A fluorescent dye to monitor superoxide production; ex/em = 490/590 nm; has been used to detect ROS and intracellular superoxide in vitro
Eurofins Discoverx PathHunter eXpress HCRTR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0258E2CP0M)
The PathHunter HCRTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orexin A) based activation of HCRTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HCRTR1 and EA-tagged β-Arrestin-2. Activation of HCRTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CALCR-RAMP2 (AMY2) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0267E2CP0M)
The PathHunter CALCR-RAMP2 (AMY2) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRL1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0264E2CP0L)
The PathHunter OPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRL1 and EA-tagged β-Arrestin-2. Activation of OPRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0258E2CP0L)
The PathHunter HCRTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orexin A) based activation of HCRTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HCRTR1 and EA-tagged β-Arrestin-2. Activation of HCRTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDNRA CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0257E2CP0L)
The PathHunter EDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of EDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRA and EA-tagged β-Arrestin-2. Activation of EDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.