General Cell Based Assays
Bioassay Systems EnzyChrom™ Acetate Assay Kit. For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.
For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism. Key Features: Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays. Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature and read. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, food, agriculture and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.13 mM.
Abcam Tissue type Plasminogen Activator Activity Assay Kit (Colorimetric, Human)
Tissue type Plasminogen Activator Activity Assay Kit (Colorimetric, Human)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Resazurin Assay Kit (Cell Viability)
Resazurin Assay Kit (Cell Viability)
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Sulfate Assay Kit sufficie
Inorganic sulfate is one of the most abundant anions in mammalian plasma. Sulfate plays important physiological roles in activating and detoxifying xenobiotics steroids neurotransmitters and bile acids. Sulfate is needed for the biosynthesis of cerebroside sulfate glycosaminoglycans and heparin sulfate. Undersulfation of cartilage proteoglycans has been associated with human inherited osteochondro-dysplasia disorders. In mammals sulfate homeostasis is regulated by the kidney. The majority of filtered sulfate is absorbed in the proximal tubules and only 5-20% of the filtered load is excreted into the urine.
Molecular Devices FLIPR Calcium 6-QF Assay Kit, Quench-Free, Sample, 96/384/1536-well
The Molecular Devices FLIPR Calcium 6-QF Sample Kit is a quench-free, mix-and-read fluorescent reagent set designed for sensitive intracellular calcium flux measurements. The high-quantum-yield dye increases signal amplitude for low-signal targets and the QF formulation enables assay multiplexing while minimizing quenching effects. The sample kit provides reagents sufficient for two microplates and supports 96-, 384-, and 1536-well formats.
- Quench-free dye formulation enables multiplexing with luminescent assays.
- High quantum yield increases signal window for low-signal targets.
- Mix-and-read protocol simplifies workflow and accelerates throughput.
- Reagents sufficient for two microplates, supporting 96-, 384-, and 1536-well formats.
- Includes component A (2 vials), component B buffer (1 bottle), and component C (2 vials).
- Reduces well-to-well variability, improving assay quality and reliability.
Cell Biolabs Inc MTT CELL PROLIFERATION ASSAY
CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring cell proliferation. Reagents are sufficient for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.
Eurofins Discoverx PathHunter eXpress BLK Activity Assay, 10-Plate Kit. (93-0882E3CP16L)
The PathHunter eXpress BLK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the BLK kinase domain. The cell line is engineered to express the catalytic domain from BLK fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the BLK catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems QuantiChrom™ Creatinine Assay Kit. For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism.
For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).
Molecular Devices Calcium Assay Kit, Fura-2 QBT, Homogeneous, No-Wash
The Fura-2 QBT Calcium sample kit is a homogeneous, mix-and-read assay designed to measure intracellular calcium mobilization using the ratiometric dye Fura-2. Proprietary quench/masking chemistry enables a no-wash protocol to reduce steps and minimize assay artifacts.
- Homogeneous mix-and-read assay for calcium mobilization.
- No-wash protocol to reduce assay steps and variability.
- Ratiometric Fura-2 dye provides excitation at 340/380 nm, emission at 510 nm.
- Sample kit format contains two plates worth of reagent.
- Compatible with plate-based fluorescence readers and FLIPR systems.
- Minimizes effects of uneven dye loading and dye leakage on results.
Molecular Devices Neurotransmitter Uptake Assay, Fluorescent, Homogeneous, 96/384-Well
The Molecular Devices Neurotransmitter Transporter Uptake Assay Kit is a homogeneous, mix-and-read fluorescent assay for measuring live-cell uptake activity of serotonin, norepinephrine, and dopamine transporters. It supports kinetic and endpoint workflows and is suitable for high-throughput screening and assay development.
- Homogeneous mix-and-read fluorescent format compatible with automation.
- Suitable for kinetic real-time measurements and endpoint assays.
- Compatible with 96-well and 384-well plate formats.
- Evaluation kit configuration includes reagents sufficient for two plates.
- Replaces radioactive uptake assays, reducing disposal and safety concerns.
Molecular Devices Cardiotoxicity Assay Kit, Fluorescence, Calcium Indicator, Bulk 10-Plate
The EarlyTox Cardiotoxicity Kit provides a fluorescence-based calcium assay for rapid detection of cardiotoxic compounds in stem cell-derived cardiomyocytes. The bulk kit supplies reagents for high-throughput screening of up to ten plates and uses a calcium-sensitive dye with masking technology to improve signal window and reduce extracellular background.
- Fluorescence-based calcium assay for cardiomyocyte function
- Bulk kit supplies reagents for up to ten plates
- Compatible with 96-, 384-, and 1536-well plate formats
- Includes loading dye, dilution buffer, and reference compounds
- Mix-and-read, no-wash homogeneous protocol
- Optimized for high-throughput fluorescence plate readers and kinetic imaging
Eurofins Discoverx PathHunter eXpress GIPR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-1095E2CP2M)
The PathHunter GIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of GIPR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GIPR and EA-tagged β-Arrestin-2. Activation of GIPR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IFNGR1/IFNGR2 Dimerization Assay, 10pl Kit. (93-1098E3CP5L)
The PathHunter IFNGR1/IFNGR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IFN-g) induced dimerization of IFNGR1/IFNGR2 receptors. This product uses EFC technology. The cell line included overexpresses IFNGR1 target protein fused to PK alongside IFNGR2 target protein fused to EA. Upon ligand-induced dimerization of IFNGR1/IFNGR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.