General Cell Based Assays
Cayman Chemical 7hydroxy CoumarIn sulfatep
A phase II metabolite of coumarin; an internal standard for coumarin metabolism
Eurofins Discoverx AssayComplete Cell Plating 24 Reagent, 100. (93-0563R24A)
AssayComplete Cell Plating 24 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress LGR5 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0526E2ACP1L)
The PathHunter LGR5 (GPR49) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. R-spondins) based activation of LGR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR5 and EA-tagged β-Arrestin-2. Activation of LGR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 24 Reagent, 500. (93-0563R24B)
AssayComplete Cell Plating 24 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx AssayComplete Cell Plating 18 Reagent, 100. (93-0563R18A)
AssayComplete Cell Plating 18 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress TrkC-P75 Functional Assay, 10-Plate Kit. (93-0531E3CP16L)
The PathHunter eXpress TrkC-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCMKLR1 CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0537E2MCP0L)
The PathHunter mCMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of mCMKLR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCMKLR1 and EA-tagged β-Arrestin-2. Activation of mCMKLR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
STEMCELL Technologies ALDHbr Assay Kit, For labeling: 6 x 10^7 cells
The ALDHbr Assay Kit is optimized for the identification and quantitation of CD34+ cells that express high levels of the enzyme aldehyde dehydrogenase (ALDH) in human cord blood samples. This kit contains both the CD34+ Cell Detection Kit and the ALDEFLUOR™ Kit. The CD34+ Cell Detection Kit contains pre-selected fluorochrome-conjugated monoclonal antibodies that have been optimized for use with the ALDEFLUOR™ Kit to detect cells that express CD34 and have high ALDH activity by flow cytometry. The CD34+ Cell Detection Kit contains anti-human antibodies APC CD34, PE CD45, and PE-Cyanine5 Glycophorin A/B, and 7-AAD viability dye.A flow cytometer equipped with a 488 nm laser and a 635 nm laser for excitation and appropriate filters for detecting APC, PE, PE-Cyanine5 and 7-AAD fluorescence is required for use.
Arbor Assays Glutathione Fluorescent Detection Kit (5 Plate)
The DetectX Glutathione (GSH) Fluorescent Detection Kit utilizes a proprietary non-fluorescent molecule, ThioStar, that covalently binds to the free thiol group on GSH to yield a highly fluorescent product.
Revvity Health Sciences Inc HTRF KinEASE detection buffer, 200 ml
HTRF KinEASE detection buffer, 200 ml
Cayman Chemical HDAC CelBased ActIvIty96
Cayman’s HDAC Cell-Based Assay Kit provides a robust and EAsy-to-use platform for studying HDAC activity modulators in whole cells. By using a cell-permeable HDAC substrate, the activity of various lysine-specific protein deacetylases can be measured in intact cells in a simple and homogenous manner. The fluorescence of the deacetylated reaction product can be analyzed using a fluorometer with an excitation wavelength of 340-360 nm and an emission wavelength of 440-460 nm. An HDAC inhibitor, trichostatin A, is included for checking specificity of the HDAC reaction. This assay parallels Cayman’s HDAC Activity Assay Kit (Item No. 10011563), which uses a nuclear extract rather than whole cells for the assay. Together, both assays will help identify potential inhibitors/activators of HDAC enzymes.
Cell Biolabs Inc Adenosine Assay Kit, 100 assays, Fluorometric, Detection Limit 1.56 μM, 96-well microplate
The Adenosine Assay Kit provides a simple and reliable fluorometric method to quantify total adenosine levels in various biological samples. It is designed for scientific researchers to investigate the roles of adenosine in energy transfer, cell signaling, and blood flow regulation. The kit is optimized for diverse research applications, allowing detection of adenosine levels as low as 1.56 μM, making it suitable for low-abundance samples.
- Accurate quantification of total adenosine in biological samples.
- High sensitivity allows detection of adenosine concentrations as low as 1.56 μM.
- Compatible with cell lysates, tissue lysates, serum, plasma, and urine samples.
- Utilizes a 96-well microplate format for efficient, high-throughput analysis.
- Comprehensive kit includes adenosine standard, assay buffer, fluorometric probe, and necessary enzymes.