General Cell Based Assays
Eurofins Discoverx PathHunter eXpress LGR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0527E2ACP1M)
The PathHunter LGR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR6 and EA-tagged β-Arrestin-2. Activation of LGR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress LGR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0527E2ACP1L)
The PathHunter LGR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR6 and EA-tagged β-Arrestin-2. Activation of LGR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TrkB-P75 Functional Assay, 2-Plate Kit. (93-0530E3CP16M)
The PathHunter eXpress TrkB-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BPS Bioscience Inc CBL-B TR-FRET ASSAY KIT
Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B) is an E3 ubiquitin-protein ligase that functions as a negative regulator of T-cell activation. The CBL-B TR-FRET Assay Kit is designed to measure CBL-B auto-ubiquitination activity in a homogeneous 384 reaction format.
Cell Biolabs Inc Total Cholesterol Assay Kit (Colorimetric), 192 assays
Cell Biolabs’ Total Cholesterol Assay Kit is a simple colorimetric assay that measures the amount of total cholesterol present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, cholesterol standards and unknown samples. Sample cholesterol concentrations are determined by comparison with a known cholesterol standard. Cholesteryl esters can be quantified by subtracting the free cholesterol values from the total cholesterol value.
Eurofins Discoverx PathHunter VIPR2 Bioassay Kit, 10-Plate. (93-0317Y2-00088)
The PathHunter VIPR2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged VIPR2 and EA-tagged β-Arrestin-2. Activation of VIPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx AssayComplete Cell Plating 5 Reagent, 500. (93-0563R5B)
AssayComplete Cell Plating 5 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Abcam Acetylcholinesterase Assay Kit (Fluorometric Red)
Acetylcholinesterase Assay Kit (Fluorometric Red)
The product is subject to the following: Abcam Restricted Use Statement
Cell Biolabs Inc CELL ADHESION ASSAY- 48 ASSAYS
Full quantitation of cell adhesion with no manual cell countingFluorometric or colorimetric detectionPlates precoated with a uniform substrate layer of a single ECM protein in each row: Collagen I, Collagen IV, Fibrinogen, Fibronectin, and Laminin.Our CytoSelect™ 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control. Just follow a simple protocol:Cells are seeded onto the substrate.Adherent cells attach, while non-adherent cells are washed away.Adherent cells are quantified on a standard plate reader or fluorometer.
Indigo Biosciences Human Insulin Receptor isoform B (INSRb) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Insulin Receptor, isoform B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against Insulin Receptor, isoform B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.