General Cell Based Assays
Eurofins Discoverx PathHunter Exendin-4 Bioassay Kit, 2-Plate. (93-0300Y2-00029)
The PathHunter Exendin-4 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exendin-4 based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress PRLHR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0302E2CP2L)
The PathHunter PRLHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PrRP) based activation of PRLHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PRLHR and EA-tagged β-Arrestin-2. Activation of PRLHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR7 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0689E3MCP5L)
The PathHunter mCXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR7 and EA-tagged β-Arrestin-2. Activation of mCXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR150 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0375E2ACP1L)
The PathHunter GPR150 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR150 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR150 and EA-tagged β-Arrestin-2. Activation of GPR150 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR65 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0428E2ACP1L)
The PathHunter GPR65 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR65 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR65 and EA-tagged β-Arrestin-2. Activation of GPR65 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Biotium Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays)
The Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells provides green/red fluorescent staining of live and dead cells, respectively. The assay employs two probes: Calcein AM that detects intracellular esterase activity and stains live cells green, and Ethidium homodimer III (EthD-III) that stains cells with compromised plasma membrane integrity red. The assay principal is applicable to most eukaryotic cell types, including adherent cells and certain tissues, but not bacteria or yeast, and can be used in place of trypan blue exclusion, 51Cr release, or similar methods for determining cell viability and cytotoxicity. The kit is suitable for fluorescence microscopy, fluorescence microplate reader, or flow cytometry. EthD-III, a superior alternative to the spectrally similar Ethidium homodimer I, has a higher DNA binding affinity with ~30% brighter fluorescence.Calcein (end-product of calcein AM hydrolysis): Ex/Em: 494/517 nm (pH 8); EthD-III: Ex/Em: 530/620 nm (with DNA).
Eurofins Discoverx AssayComplete Cell Plating 36 Reagent, 500. (93-0563R36B)
AssayComplete Cell Plating 36 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx AssayComplete Cell Plating 3 Reagent, 500. (93-0563R3B)
AssayComplete Cell Plating 3 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx AssayComplete Cell Plating 27 Reagent, 500. (93-0563R27B)
AssayComplete Cell Plating 27 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress DRD3 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0591E3CP0L)
The PathHunter DRD3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD3 and EA-tagged β-Arrestin-2. Activation of DRD3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Aat Bioquest Amplite™ Fluorimetric Catalase Assay Kit *Red Fluorescence*
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen. The enzyme is concentrated in the peroxisome subcellular organelles.
Abcam Cambinol, SIRT1/2 inhibitor, 5MG
MW 360.4 Da, Purity >98%. SIRT1/2 inhibitor (IC₅₀ values are 56 and 59 μM at SIRT1 and SIRT2 respectively). Class III HDAC inhibitor, with no inhibition of class I and II. Promotes G₂ cell cycle arrest. Antitumour activity in vitro and in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Brookfield Engineer Labs VIAL TESTING ASSAY KIT 10PK
DescriptionGlass Sample bottles with metal cap and septa. Compatible with Vapor Pro instruments CT-3100(-L) and CT-4200XL. Includes 10 glass sample bottles.
Eurofins Discoverx PathHunter GHSR Bioassay Kit, 2-Plate. (93-0242Y3-00141)
The PathHunter GHSR Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Bioassay Systems QuantiChrom™ Boron Assay Kit. Quantitative determination of boron in agricultural and environmental samples.
For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.