General Cell Based Assays
Aat Bioquest iFluor™ 647 azide
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
Cayman Chemical Hydrogen Peroxide Assay Kit, 480 Well
Cayman’s Hydrogen Peroxide Assay Kit provides fluorometric or colorimetric methods for the quantification of extracellular H2O2 produced by cultured cells. In the presence of horseradish peroxidase (HRP), H2O2 reacts stoichiometrically with an HRP substrate, ADHP, to produce the compound resorufin. Resorufin fluorescence can be easily quantified at excitation and emission wavelengths of 530-540 and 585-595 nm, respectively. Alternatively, the absorbance of resorufin can be measured at 570 nm. Catalase, an H2O2 scavenger, is included in the kit for verifying the specificity of the assay. ROS, such as H2O2 and superoxide, are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets. This kit provides a valuable method for immunologists to assess the capacity of immune cell killing using ROS. This assay offers the option to measure absorbance or fluorescence. It is at the user’s.
Eurofins Discoverx PathHunter eXpress BDKRB1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0639E2CP2M)
The PathHunter BDKRB1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. bradykinin) based activation of BDKRB1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB1 and EA-tagged β-Arrestin-2. Activation of BDKRB1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
