General Cell Based Assays
Eurofins Discoverx cAMP Hunter™ Human MIP-3 beta (CCR7) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MIP-3 beta (CCR7) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CCR7 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 10-Plate Kit. (93-0875E3CP16L)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human BMP-6 (BMPR1B/ACVR2B) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human BMP-6 (BMPR1B/ACVR2B) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of BMPR1B/ACVR2B. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PTGER3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PTGER3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PTGER3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Human TNF-B ASY 25 PL
The Meso Scale Discovery U-PLEX Human TNF-beta Assay Kit is a multiplex immunoassay that measures human TNF-beta in serum plasma cell culture supernatants and other biological samples It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay can be run on Meso Scale Discovery instrumentation including the QuickPlex SQ 120 QuickPlex SQ 120i MESO QuickPlex SQ 120 and V-PLEX Plus The kit includes a 96-well plate coated with capture antibodies detection antibodies calibrators and ancillary reagents needed to perform the assay
Eurofins Discoverx PathHunter eXpress TrkC-P75 Functional Assay, 10-Plate Kit. (93-0531E3CP16L)
The PathHunter eXpress TrkC-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR109B CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0791E2CP2L)
The PathHunter GPR109B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 3-hydroxyoctanoic acid) based activation of GPR109B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109B and EA-tagged β-Arrestin-2. Activation of GPR109B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mADCYAP1R1 (PAC1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0594E2MCP0L)
The PathHunter mADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of mADCYAP1R1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mADCYAP1R1 and EA-tagged β-Arrestin-2. Activation of mADCYAP1R1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress RXFP3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0692E2CP2L)
The PathHunter RXFP3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged RXFP3 and EA-tagged β-Arrestin-2. Activation of RXFP3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR142 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0413E2ACP1L)
The PathHunter GPR142 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR142 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR142 and EA-tagged β-Arrestin-2. Activation of GPR142 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cellular Dynamics International iCell Microglia Media Kit, 50 ml iCell Glial Base Medium, 0.5 ml iCell Microglia Supplement A (100x), 0.5 ml iCell Microglia Supplement B (100x), 1 ml iCell Neural Supplement C (50X)
The iCell Microglia Media Kit is a specialized cell culture medium tailored for the optimal growth and maintenance of human iPSC-derived microglia. It provides a consistent in vitro environment, essential for research in microglial biology and neurodegenerative diseases. This media kit solves the challenges faced with primary human microglia by ensuring reproducible results for various experiments related to neuroinflammation and drug discovery.
- Supports growth of iPSC-derived human microglia.
- Includes multiple supplements for enhanced performance.
- Stores complete medium at 4°C for up to 2 weeks.
- Can be aliquoted for single-use storage at -20°C for up to 3 months.
- Recommended culture conditions: 37°C and 5% CO2.
- Designed for research purposes only, focusing on neurodegeneration and drug discovery.
- Maintains relevant microglial markers and physiological functions.
Eurofins Discoverx PathHunter Human BDKRB2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human BDKRB2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure BDKRB2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx SPRINTer™ Human BRD4 Targeted Protein Degradation Cell-Based Assay Kit (HCT-116)
The SPRINTer™ Human BRD4 Targeted Protein Degradation Stable Cell Line Assay (HCT-116) contains a stable clonal cell line used for monitoring drug-induced protein turnover of BRD4, supporting TPD studies. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR139 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR139 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR139 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR152 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human GPR152 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure GPR152 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.