General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR152 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human GPR152 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure GPR152 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx HitHunter cGMP Assay, 10,000 dp (384-well). (90-0039L)
The HitHunter cGMP Assay is a detection kit offering a robust, sensitive and easy-to-use assay to measure intracellular cGMP levels. This product uses EFC technology, and contains ED-tagged cGMP, anti-cGMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cGMP, the anti-cGMP antibody will be sequestered by binding to this untagged cGMP, allowing for EFC between the tagged cGMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit may be used with Eurofins DiscoverX cell lines as well as cell lines from other sources. This detection kit contains reagents for 25 plates (384 well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter eXpress NTSR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0671E3CP5M)
The PathHunter NTSR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NTSR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged NTSR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting NTSR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR7 Activated GPCR Internalization Assay, 10pl Kit. (93-0738E3CP27L)
The PathHunter CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR7 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ErbB4/ErbB4 Dimerization Assay, 10pl Kit. (93-0961E3CP5L)
The PathHunter ErbB4/ErbB4 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. neuregulin-1 Beta 2) induced dimerization of ErbB4/ErbB4 receptors. This product uses EFC technology. The cell line included overexpresses ErbB4 target protein fused to PK alongside ErbB4 target protein fused to EA. Upon ligand-induced dimerization of ErbB4/ErbB4, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTAFR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0236E2CP0M)
The PathHunter PTAFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTAFR and EA-tagged β-Arrestin-2. Activation of PTAFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx Calcium No WashPLUS, 20 plates (384-well). (90-0091L)
The Calcium No WashPLUS detection kit is intended for use with cells expressing receptors that signal through calcium. The kit contains a calcium-sensitive dye which assays intracellular calcium levels upon activation of a Gαq-coupled GPCR. Activation of the GPCR leads to release of intracellular calcium, and a detectable increase in fluorescent signal. This detection kit contains reagents for assaying 20 plates. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/cell-line/calcium-no-washplus-90-0091l.
Eurofins Discoverx PathHunter eXpress IL23R/IL12RB1 Dimerization Assay, 2pl Kit. (93-1007E3CP0M)
The PathHunter IL23R/IL12RB1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-23) induced dimerization of IL23R/IL12RB1 receptors. This product uses EFC technology. The cell line included overexpresses IL23R target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL23R/IL12RB1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human IL-17RB ASY
The R-PLEX Human IL-17RB Assay Kit from Meso Scale Discovery is a multiplex immunoassay that enables the simultaneous measurement of IL-17RB in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity up to a 7-log dynamic range and requires low sample volume The kit includes a 96-well plate pre-coated with capture antibodies detection antibodies calibrators and controls for the quantitative analysis of IL-17RB This assay allows for efficient and reproducible quantification of IL-17RB levels
Eurofins Discoverx ADP Hunter Plus, 500 (50,000 dp, 384-well). (90-0083XL)
The ADP Hunter Plus kit is a detection kit for the measurement of ADP produced as a product of kinase activity. It is used to detect substrate-induced activity of purified or semi-purified kinases. ADP produced by the kinase reacts with intermediate factors to produce a fluorescent dye precursor, which generates signal when combined with peroxide and peroxidase. The kit uses an end-point read. This detection kit contains reagents for 125 plates (384-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Cayman Chemical GlutathIon asy KIt 96Wel
Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative protocol. The GSH Assay can be used for plasma, tissue samples, and cultured cells with minimal sample processing.
Ethos Biosciences Exocell DTNB Thiol Oxidative Stress Assay, Ethos
The Ethos Biosciences DTNB (Ellman’s) Thiol Oxidative Stress Assay Kit is designed to measure free thiol groups that arise due to oxidative stress, which is an underlying component of acute and chronic kidney disease. Our kit uses Ellman’s Reagent otherwise known as DTNB or 5-5′-dithiobis [2-nitrobenzoic acid] which reacts with the disulfide bond in a sample to produce 2-nitro-5-thiobenzoic acid. Under conditions of oxidative stress, free thiols decrease and disulfides increase. Determination of the free thiol concentration reflects the ability to detoxify lipid (and other) peroxides. Aliquots of standard or sample are reacted with rehydrated Ellman’s reagent (DTNB) in microtiter wells. Our DTNB (Ellman’s) Thiol Oxidative Stress Assay kit contains all of the components and reagents you need to conduct a 96 well plate chemical assay for thiol groups of any species in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…)
![Medchemexpress LLC 11-(2-fluoropyridin-4-yl)-4,8,10-triazatricyclo[7.4.0.02,7]trideca-1(9),2(7),3,5,10,12-hexaene | 2173361-80-1 | 99.9% | C15H9FN4 | 10MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000260229.png-250.jpg)
Medchemexpress LLC 11-(2-fluoropyridin-4-yl)-4,8,10-triazatricyclo[7.4.0.02,7]trideca-1(9),2(7),3,5,10,12-hexaene | 2173361-80-1 | 99.9% | C15H9FN4 | 10MG
Tau tracer 2 is a small-molecule PET tracer developed for imaging aggregated tau protein in neurodegenerative disease research. It binds selectively to tau aggregates and is used in preclinical and diagnostic studies to assess regional tau pathology with PET imaging.
- High affinity for aggregated tau enabling sensitive PET imaging.
- Suitable for preclinical and diagnostic research applications.
- High chemical purity suitable for analytical and radiolabeling workflows.
- Supplied in small-mass packages for tracer synthesis and testing.
- Characterized by molecular formula C15H9FN4 and CAS 2173361-80-1.
- Datasheet available with analytical and handling information.
STEMCELL Technologies Wheat Germ Agglutinin WGA i
Fluorescent dye conjugates for staining cell membranes of gram-positive bacteria, mammalian cells, and yeast bud scars - 1000 Ug
Eurofins Discoverx PathHunter eXpress mGIPR U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0846E3MCP5M)
The PathHunter mGIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of mGIPR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGIPR and EA-tagged β-Arrestin-2. Activation of mGIPR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.