General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CXCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0248E2CP2L)
The PathHunter CXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR7 and EA-tagged β-Arrestin-2. Activation of CXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human GRPR Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human GRPR Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure GRPR (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human NTSR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human NTSR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure NTSR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx InCELL Hunter eXpress MEK1 Kinase Binding Assay, 2pl Kit. (96-0040E15CP0M)
The InCELL Hunter eXpress MEK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of MEK1 via detection of MEK1 levels. This product uses EFC technology. The cell line included overexpresses MEK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human UTR2 Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human UTR2 Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure UTR2 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Aat Bioquest iFluor™ 647 azide
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
