General Cell Based Assays
Dojindo Molecular Technologies Inc NAD/NADH Assay Kit-WST (100TESTS), enables quantification of the amount of total NAD+/NADH, NAD+ and NADH in cells and measurement of their ratio.
Recently, it has become clear that Sirtuin is linked to longevity and plays a role in NAD+ level regulation. Also Sirtuin has been recognized as a marker necessary to understanding biological states, such as obesity & diabetes, as well as cellular differentiation. Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of their ratio. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Cayman Chemical T16A Inh-A01 10mg
An aminophenylthiazole that inhibits transient TMEM16A-mediated chloride currents with an IC50 value of ~1 µM; blocks CaCC activity in vascular smooth muscle cells and relaxes mouse and human blood vessels
Eurofins Discoverx PathHunter Human Apelin (APJ Receptor) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human Apelin (APJ Receptor) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AGTRL1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Sigma Aldrich Fine Chemicals Biosciences Cholesterol Quantification
Cholesterol is a lipid that constitutes 20-25% of the structural components of cell membranes. Cholesterol presence assure membrane structural fluidity. Cholesterol also plays a role as an intermediate in several hormones and bile acid biosynthesis and vitamin D. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane. The major sites of cholesterol synthesis are the small intestine and liver. Cholesterol circulates in the blood as both the free acid and as cholesterol esters. Increased cholesterol levels are associated with the development of atherosclerosis and cardiovascular diseases. Thus serum cholesterol regulation has an important therapeutic role.The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol cholesteryl esters or total cholesterol (which constitutes both free cholesterol and chole
Abcam Cerivastatin sodium, HMG-CoA reductase inhibitor, 25MG
MW 481.5 Da, Purity >98%. Potent HMG-CoA reductase inhibitor (Ki = 13 nM). Shows higher potency than Lovastatin (ab120614). Enantiomerically pure pyridine derivative. Reduces plasma cholesterol levels in vivo. Orally active.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical LDL Uptake CellBased Assay Ki
LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman Chemical’s LDL Uptake Cell-Based Assay employs a preparation of human LDL conjugated to Dylight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific antibody and a Dylight™ 488-conjugated secondary antibody are included in the kit for identifying the distribution of LDL receptors. The kit provides a convenient tool for studying LDL uptake and its regulation at the cellular level.
Bioassay Systems EnzyChrom™ Creatine Kinase Assay Kit. For quantitative determination of creatine kinase activity and evaluation of drug effects on CK activity.
For quantitative determination of creatine kinase (CK) activity and evaluation of drug effects on CK activity. Key Features: Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 U/L.
Eurofins Discoverx cAMP Hunter eXpress PTHR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0057E2CP2L)
The cAMP Hunter PTHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. parathyroid hormone, TIP 39) based activation of PTHR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR2, a naturally Gs coupled GPCR. Upon activation of PTHR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Miltenyi Biotec, Inc. mIFN-g Secr.Assay Enrichm.&Det.Kit(PE)
Isolation and detection of mouse IFN-γ–secreting cells.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Eurofins Discoverx PathHunter Human PTGER2 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human PTGER2 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure PTGER2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Montana Molecular Green Downward cADDis cAMP sensor Big Sky BacMam 5mL
Detect cAMP signaling kinetics in living cells with the Green Downward cADDis fluorescent cAMP assay. Decrease in fluorescence indicates increase in cAMP. Packaged in Big Sky BacMam, for enhanced expression in HEKs, CHOs, and many other mammalian cell types and iPSCs. This is an update to product D0200G, with improved expression efficiency from Big Sky BacMam.
Cell Biolabs Inc Adenosine Assay Kit, 100 assays, Fluorometric, Detection Limit 1.56 μM, 96-well microplate
The Adenosine Assay Kit provides a simple and reliable fluorometric method to quantify total adenosine levels in various biological samples. It is designed for scientific researchers to investigate the roles of adenosine in energy transfer, cell signaling, and blood flow regulation. The kit is optimized for diverse research applications, allowing detection of adenosine levels as low as 1.56 μM, making it suitable for low-abundance samples.
- Accurate quantification of total adenosine in biological samples.
- High sensitivity allows detection of adenosine concentrations as low as 1.56 μM.
- Compatible with cell lysates, tissue lysates, serum, plasma, and urine samples.
- Utilizes a 96-well microplate format for efficient, high-throughput analysis.
- Comprehensive kit includes adenosine standard, assay buffer, fluorometric probe, and necessary enzymes.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD2(1) Bromodomain Assay, 10-plate Kit. (96-0004E1CP0L)
The InCELL Hunter eXpress HEK 293 BRD2(1) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD2(1) via detection of BRD2(1) levels. This product uses EFC technology. The cell line included overexpresses BRD2(1) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress VIPR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0317E2CP0L)
The PathHunter VIPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged VIPR2 and EA-tagged β-Arrestin-2. Activation of VIPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DRD1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0590E2CP2M)
The PathHunter DRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD1 and EA-tagged β-Arrestin-2. Activation of DRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.