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Gel Shift Assays

Gel shift assays employ affinity electrophoresis techniques to study protein–DNA and protein–RNA interactions. Ideal for determining if a protein or protein mixture is capable of binding to a target DNA or RNA sequence.
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1

Invitrogen™ AP2 gamma Recombinant Rabbit Monoclonal Antibody (K01_2L40)
GREENER_CHOICE

Rabbit Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Target Species Human
Host Species Rabbit
Conjugate Unconjugated
Applications Immunohistochemistry (Paraffin),Western Blot,Immunocytochemistry
Form Liquid
Gene Accession No. Q92754
Isotype IgG
Concentration 1 mg/mL
Antigen AP2 gamma
Gene Symbols TFAP2C
Regulatory Status RUO
Purification Method Protein A/G
Gene Alias AA409384; activating enhancer binding protein 2 gamma; activating enhancer-binding protein 2 gamma; activator protein 2 gamma; AP-2.2; AP2gamma; AP2-GAMMA; ERF1; estrogen receptor factor 1; hAP-2g; stimulated by retinoic acid gene 2; Stra2; Tcfap2c; TFAP2C; TFAP2G; Transcription factor AP-2 gamma; transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma); transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma); transcription factor AP-2, gamma; transcription factor ERF-1
Gene TFAP2C
Product Type Antibody
Gene ID (Entrez) 7022
Formulation 50mM tris glycine with 0.05% BSA, 40% glycerol, 150mM NaCl and 0.01% sodium azide; pH 7.4
Immunogen A synthetic peptide of human AP2 gamma/TFAP2C.
Classification Recombinant Monoclonal
Primary or Secondary Primary
Clone K01_2L40
2

MOLECULAR DEPOT LLC Gel Shift 5X - 4 /500 uL

Gel Shift Binding Buffer 5X is a highly optimized reproducible buffer for use in Gel Electrophoresis Mobility Shift Assay (EMSA) and similar applications Its proprietary composition counteracts non-specific interactions and results in sharp EMSA gel profiles This product is supplied as a clear solution aliquoted in four vials 500 uL each For in vitro research use only

ENCOMPASS_PREFERRED
3

HEIDELBERG ENGINEERING INC
SDP

NC3984684 OCT ANGIOGRAPHY OCTA SHIFT

ENCOMPASS
4

Biolegend Purified anti-TFAP2A [W20235D]; Isotype: Rat IgG2a, κ; Reactivity: Human; Apps: WB, ICC, ICFC, IHC-P; Size: 25 μg

Purified anti-TFAP2A [W20235D]; Isotype: Rat IgG2a, κ; Reactivity: Human; Apps: WB, ICC, ICFC, IHC-P; Size: 25 μg

ENCOMPASS_PREFERRED
5

Nanotemper Technologies Inc SPECTRAL SHIFT OPTIMIZED PROTE
SDP

NC3791715 SPECTRAL SHIFT OPTIMIZED PROTE

ENCOMPASS
6

Enzo Life Sciences PROTEOSTAT Thermal shift stability assay kit (1x400tests)
SDP

Accelerated screening for protein stability as a function of pH,  ionic strength, and concentration. The PROTEOSTAT Thermal shift stability assay kit includes a fluorescent dye which detects protein aggregation, so it can be used to monitor protein stability under systematic thermal stress conditions. From the thermal shift assay, a temperature at which the bulk of the protein becomes aggregated can readily be identified. The aggregation temperature is an indicator of protein stability and can be used to optimize conditions that minimize protein aggregation as well as to identify ligands that bind and confer structural stability to a protein of interest. Use the related PROTEOSTAT Protein Refolding and Aggregation Sensing Kit (Prod. No. ENZ-51040) to identify optimal protein refolding conditions. Conditions that increase the aggregation temperature, increase the stability of the protein. Contents: PROTEOSTAT TS Detection Reagent. β-Lactoglobulin control. 10X Assay Buffer

ENCOMPASS_PREFERRED
7

LICOR Odyssey EMSA Kit

Mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.The Odyssey EMSA Kit, coupled with IRDye 700 EMSA oligonucleotides, is an excellent alternative method to radioisotopic and chemiluminescent detection methods for EMSA analysis and visualization1, 2. Using IRDye EMSA reagents, assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even need to be removed from the glass plates for imaging.

ENCOMPASS_PREFERRED
8

Biotium BIOTIUM INC

NC3943445 PROTEIN THERMAL SHIFT KIT

ENCOMPASS_PREFERRED