Protein Purification Reagents and Kits
Hampton Research HR2-998-40/PEG/Ion 2 Screen #40 (H04), 185 ml - (0.03 M Citric acid, 0.07 M BIS-TRIS propane)/pH 7.6, 20% w/v Polyethylene glycol 3,350
HR2-998-40/PEG/Ion 2 Screen #40 (H04), 185 ml - (0.03 M Citric acid, 0.07 M BIS-TRIS propane)/pH 7.6, 20% w/v Polyethylene glycol 3,350. Please note items from this supplier are non-returnable.
Hampton Research HR2-998-38/PEG/Ion 2 Screen #38 (H02), 185 ml - (0.05 M Citric acid, 0.05 M BIS-TRIS propane)/pH 5.0, 16% w/v Polyethylene glycol 3,350
HR2-998-38/PEG/Ion 2 Screen #38 (H02), 185 ml - (0.05 M Citric acid, 0.05 M BIS-TRIS propane)/pH 5.0, 16% w/v Polyethylene glycol 3,350. Please note items from this supplier are non-returnable.
Active Motif Histone Extraction Kit
The Histone Extraction Kit provides a simple method to acid precipitate histones from cell culture, tissue and primary cells (e.g. T-cells) while preserving histone post-translational modifications. The highly basic nature of histone proteins often precludes them from being isolated using traditional methods for protein extraction. Instead, histones must be isolated under acidic conditions to retain solubility. The inclusion of inhibitors helps to preserve histone modifications such as acetylation, methylation and phosphorylation. Each Histone Extraction Kit contains enough reagents for extractions from 2 x 96-well plates (192 rxns), or 100 rxns from cultured cell lines and tissues. Kits include buffers, protease inhibitors, phosphatase inhibitors, deacetylase inhibitors and optimized protocols for efficient recovery of core histones (H2A, H2B, H3 & H4). For more complete information, please visit the Active Motif website.
Bioworld Zn-IMAC 5 x 1mL Columns, 5 Column
Zn-immobilized metal affinity chromatography (IMAC) columns consist of Zn-Chelated Separoporematrix conveniently prepacked spin columns for gravity flow chromatography.Zn-IMAC Columns separate proteins via their differences in affinity toZn2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.Technical SpecificationsImminodiacetate immobilized matrix charged withZn metal ions.Chelating group coupling method: epoxy activationMatrix: cross-linked Separopore 4BParticle size (range): 50-170µm Metal ion capacity: > 20µmol Zn2+/ml gelSpacer arm: 14 atompH stability: 3-12Binding Capacity: Approx. 40 mg His-tagged protein/ml mediumSupplied as suspension in 20% ethanol.
Hampton Research HR2-984-37/PEGRx 2 #37 (H01), 185 ml - 0.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 8,000
HR2-984-37/PEGRx 2 #37 (H01), 185 ml - 0.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 8,000. Please note items from this supplier are non-returnable.
Hampton Research Silver Bullets, 0.5ml
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization. Please note items from this supplier are non-returnable.
Mayflower Bioscience Exo-spin, 24 columns
The EX03 Exo-spin™ kit only contains pre-packed and equilibrated ready-to-use SEC columns as the precipitation step might not be recommended for some applications (e.g. mass spectrometry) or not needed for small volumes of sera sample. The EX03 Exo-spin™ kit has been developed to process any sample smaller than 100 µl. Plasma contains much higher protein levels than sera and can only be processed directly using the EX02 or EX04 Exo-spin™ kit. EX03 Exo-spin™ kit may be used to clean-up samples which have been purified by other methods, such as precipitation and ultracentrifugation, in order to remove proteins and smaller molecules. Exo-spin™ columns are also very effective for the removal of excess dye in fluorescently labelled exosome samples.
Hampton Research HR6-148/1.5 mm Quartz Capillary - 25 pack
HR6-148/1.5 mm Quartz Capillary - 25 pack. Please note items from this supplier are non-returnable.
Profoldin Colorimetric DTT Assay Kit
The Colorimetric DTT Assay Kit (Catalog No. DTT100C) measures light absorbance at 590 nm. It is based on the strong reducing property of DTT. The assay is not interfered by phosphate buffer, mono thio compounds such as cysteine or chelators. It is interfered by NADH or NADPH.
Agilent Technologies AdvanceBio total sialic acid quantitation kit, 48/pk
Rapid quantitation of total sialic acid by fluorescence or absorbance detection, either free or released from intact glycoproteins. Automatable, 96-well microplate format with fluorescence detection. Broad range of detection, 40 pmol - 1,000 pmol. Sample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing. Enzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, degradation of sialic acid.
Tosoh Bioscience LLC TSKGEL OCTYL-80TS 4.6X250
Column, Reversed Phase Chromatography; Tosoh TSKgel Octyl(C8)-80Ts; Media: Silica; Particle: 5 micrometer; Pore Size: 80 angstrom; 4.6mm I.D. x 25cm Length; Stainless Steel - Standard
AdipoGen Tim-4 msFc hrecBiotin
Protein. The extracellular domain of mouse Tim-4 aa 22-279 is fused to the N-terminus of the Fc region of human IgG1. Source CHO cells. Endotoxin content <0.05EU/µg protein LAL test Lonza. Lyophilized from 0.2µm-filtered solution in PBS. Purity >95% SDS-PAGE. Extracellular vesicles EVs are released by a variety of cells into the cellular microenvironment and have the natural ability of delivering different cargos and carry bioactive molecules such as non-coding RNA, miRNAs, genomic DNA, lipids, growth factors and signaling molecules. EVs can be divided into exosomes 30-100nm, microvesicles 100-1000nm and apoptotic bodies >1000nm. EVs play substantial roles not only in the regulation of normal physiological processes but also in disease pathogenesis and their cargo reflects the status of parental cells at the time of secretion. Various studies are currently being conducted to develop therapeutic and diagnostic methods targeting or utilizing EVs.
Tosoh Bioscience LLC TSKGEL ODS-120T 4.6X250
Column, Reversed Phase Chromatography; Tosoh TSKgel ODS-120T; Media: Silica; Particle: 5 micrometer; Pore Size: 120 angstrom; 4.6mm I.D. x 25cm Length; Stainless Steel
Protifi LLC S-Trap mini kit (10x 100 – 300 ug)
S-Trap sample processing is now available in a full kit format. The kit includes lysis, wash, and digestion buffers. The user must supply HPLC grade MeOH.
Cayman Chemical DYKDDDDK PeptIDtrIfluoROC 5mg
A octapeptide fusion tag; has commonly been used as an epitope tag on recombinant proteins to facilitate their detection and purification by DYKDDDDK-specific antibodies in vitro and in vivo