Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ Human CD2 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD2 Positive Selection Kit II is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells.This product replaces the EasySep™ Human CD2 Positive Selection Kit (Catalog #18657) for even faster cell isolations.
Cayman Chemical MItoCheck MItochondrIalTIs 1ea
When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman’s MitoCheck Mitochondria (Tissue) Isolation Kit includes a complete package of reagents and step-by-step instructions for isolating coupled mitochondria* from freshly harvested mammalian heart, liver, or kidney tissue through a process of differential centrifugation in isotonic buffers. *Coupled mitochondria are defined as mitochondria capable of phosphorylating ADP at the F1FO-ATP synthase (Complex V) through the utilization of a proton (H+) gradient, which is generated by the electron transport chain (ETC) in the presence of appropriate substrates (e.g., succinate) and in the absence of uncouplers and mitochondrial inhibitors.
Miltenyi Biotec, Inc. M CD8a-FITC, human, REA1024, 200ul
Recombinant antibody for the detection of CD8a+ cells.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Miltenyi Biotec, Inc. CD4-FITC
VIT4 reacts with human CD4. The fluorescently labeled CD4 antigen can be detected by flow cytometry..
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
STEMCELL Technologies RosetteSep™ Human Granulocyte Depletion Cocktail, For processing: 40 mL whole blood processed
The RosetteSep™ Human Granulocyte Depletion Cocktail is designed to deplete granulocytes from whole blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The granulocyte-depleted fraction is present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Mouse CD4+CD62L+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). Then CD4+CD62L+ cells, pre-labeled with CD62L PE, are selected using the EasySep™ PE Selection Cocktail. The desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet.For the isolation of mouse naïve CD4+ T cells in as little as 15 minutes by negative selection, we recommend using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (19765).
STEMCELL Technologies RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, For processing: 200 mL whole blood processed
The RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36 is designed to enrich circulating epithelial tumor cells from fresh whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD16, CD19, CD36, CD38, CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified tumor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.This cocktail is compatible with small-cell carcinoma samples, including lung cancer samples. If you are working with breast cancer samples, use the RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56 (Catalog #15137).
STEMCELL Technologies EasySep™ Mouse CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-CD8+ T cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.This product replaces the EasySep™ Mouse CD8+ T Cell Enrichment Kit (Catalog #19753) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human EpCAM Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
Miltenyi Biotec, Inc. MACSxpress Buffy Coat Starting Kit
Kit containing MACS Equipment and Reagent for magnetic cell separation directly from buffy coat using MACSxpress Beads
STEMCELL Technologies EasySep™ Mouse CD8a Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD8a Positive Selection Kit II is designed to isolate CD8a+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD8a and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD8a Positive Selection Kit (Catalog #18753) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
STEMCELL Technologies EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative followed by positive selection. CD4+ T cells are first enriched by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells are then positively selected from the pre-enriched cells using EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail.
CARTERRA INC CMD50M CHIP
NC3831894 CMD50M CHIP