Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
Isolate highly purified CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh whole blood prior to chimerism testing. Desired cells are targeted with tetrameric antibody complexes recognizing CD33, CD66b, and dextran-coated magnetic particles, and separated without the use of columns through immunomagnetic positive selection using an EasySep™ magnet. Cells of interest remain in the tube while unwanted cells are poured off. This product can be used with RoboSep™ to automate immunomagnetic cell isolation. Visit www.EasySep.com for more information.
Agilent Technologies ADVANCEBIO SIALIC ACID PROFILI
AdvanceBio Sialic Acid Profiling and Quantitation
Abcam Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation
Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation
The product is subject to the following: Abcam Restricted Use Statement
Biolegend LEGENDplex™ Mouse L-selectin Capture Bead B5, 13X; Reactivity: Mouse; Apps: Multiplex - Component; Size: 100 tests (270 μL)
LEGENDplex™ Mouse L-selectin Capture Bead B5, 13X; Reactivity: Mouse; Apps: Multiplex - Component; Size: 100 tests (270 μL)
AdipoGen KillerTRAIL Soluble hrec
Protein. The extracellular domain of human TRAIL aa 95-281 is fused at the N-terminus to a His-tag and a linker peptide. Source E. coli. Endotoxin content <0.01EU/µg purified protein LAL test Lonza. Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT. Binds to human and mouse TRAIL receptors and osteoprotegerin OPG. Purity >98% SDS-PAGE. TNF-related apoptosis-inducing ligand TRAIL Apo2LCD253 TNFSF10 is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor TNF superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors TRAIL-R1 DR4 Apo2CD261 TNFRSF10A, TRAIL-R2 DR5 KILLER TRICK2ATRICK2B CD262 TNFRSF10B, TRAIL-R3 DcR1LIT TRID CD263 TNFRSF10C, TRAIL-R4 DcR2TRUNDD CD264 TNFRSF10D, and osteoprotegerin OPG OCIF TNFRSF11B.
AdipoGen DNER ED msFc h rec
Protein. Signal peptide and extracellular domain of mouse DNER aa 1-640 are fused at the C-terminus to the Fc portion of human IgG. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS. Purity >85% SDS-PAGE. DNER is an activator of the Notch1 pathway. It mediates neuron-glia interaction during astrocytogenesis.
STEMCELL Technologies RoboSep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative followed by positive selection. CD4+ T cells are first enriched by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells are then positively selected from the pre-enriched cells using EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail.
Genscript Corporation DNAM-1/CD226Biotin His Av
DNAX accessory molecule-1 (DNAM-1) also known as CD226 is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily DNAM-1 mediates cellular adhesion to other cells bearing its ligands CD112 and CD155 and cross-linking DNAM-1 with antibodies causes cellular activation Furthermore DNAM-1 can interact with PVR and PVRL2
STEMCELL Technologies Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit, For processing: 500 mL cord blood processed
Includes RosetteSep™ human cord blood progenitor kit and HetaSep™
STEMCELL Technologies EasySep™ Human CD138 Positive Selection Kit II
The EasySep™ Human CD138 Positive Selection Kit II is designed to isolate CD138+ (syndecan-1) cells from fresh or previously frozen bone marrow or peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. This cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).This product is an improved version of the EasySep™ Human CD138 Positive Selection Kit (Catalog #18357) that provides highly purified cells using a shorter protocol.
Mayflower Bioscience Exo-spin, 48 columns, 500 ml buffer
The EX03 Exo-spin™ kit only contains pre-packed and equilibrated ready-to-use SEC columns as the precipitation step might not be recommended for some applications (e.g. mass spectrometry) or not needed for small volumes of sera sample. The EX03 Exo-spin™ kit has been developed to process any sample smaller than 100 µl. Plasma contains much higher protein levels than sera and can only be processed directly using the EX02 or EX04 Exo-spin™ kit. EX03 Exo-spin™ kit may be used to clean-up samples which have been purified by other methods, such as precipitation and ultracentrifugation, in order to remove proteins and smaller molecules. Exo-spin™ columns are also very effective for the removal of excess dye in fluorescently labelled exosome samples.