Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II, For processing: 60 mL whole blood or bone marrow processed
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
Sino Biological Human CD4 / LEU3 Protein (aa 1-208, His Tag), Endotoxin-Free 100µg
A DNA sequence encoding the human CD4 (NP_000607.1) N-terminal two domains (D1D2) (Met 1-Ser 208) was fused with a polyhistidine tag at the C-terminus.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Human Monocyte Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Enrichment Kit is designed to isolate monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to prevent nonspecific binding of monocytes. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which CD16+ cells are not removed, we recommend the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058). For even faster cell isolations, we recommend the new EasySep™ Human Monocyte Isolation Kit (19359) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-Monocyte-Isolation-Kit.aspx) which isolates cells in as little as 12.5 minutes.
STEMCELL Technologies EasySep™ Mouse Plasmacytoid DC Isolation Kit, For processing: 2 x 10^9 cells
The EasySep™ Mouse Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-pDCs. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies Mitochondrial Isolation Kit, Size: 50 Tests
Conveniently enrich the mitochondrial or cytosolic subcellular fractions from mammalian cells or tissues using the Mitochondrial Isolation Kit. This kit offers two quick options to isolate the mitochondrial subcellular fraction and has been verified for use with western blot analysis. The first option is a reagent-based method for processing up to six samples concurrently. The second option utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both options use a microcentrifuge-based method to separate the mitochondrial fraction from the cytosolic fraction while preserving their biological activity. This separation is a valuable tool for studying apoptosis and signaling events between the two fractions. For more information on the Mitochondrial Isolation Kit, visit www.stemcell.com.
STEMCELL Technologies Complete RosetteSep™ Human Cord Blood Progenitor Enrichment Kit, For processing: 500 mL cord blood processed
Includes RosetteSep™ human cord blood progenitor kit and HetaSep™
STEMCELL Technologies RoboSep™ Tube Kit (9 Plastic Tubes + Tip Head Protector), Size: 1 Kit
RoboSep™ Tube Kit is a supplementary product for the operation of the RoboSep™-S (/products/robosep-s.html) instrument .For more information on using RoboSep™ to perform fully automated cell separation, please see our RoboSep™ overview (/products/brands/robosep-cell-separation-instrument.html).
STEMCELL Technologies EasySep™ Human Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553). For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653). (https://www.stemcell.com/products/easysep-release-human-biotin-positive-selection-kit.html)
STEMCELL Technologies EasySep™ Mouse SCA1 Positive Selection Kit, For processing: 2 x 10^9 cells
The EasySep™ Mouse SCA1 Positive Selection Kit is designed to isolate SCA1+ cells from single-cell suspensions of bone marrow by positive selection. The cocktail contains a combination of PE-labeled antibodies that is directed against SCA1, which are then recognized by antibody complexes that are directed against PE and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This kit replaces the EasySep™ Mouse SCA1 Biotin Positive Selection Kit (Catalog #18856) for even faster cell isolations.
Genscript Corporation CD8 alpha/CD8A His Human
CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I favoring lipid raft partitioning of TCR and inducing intracellular signaling CD8 alpha is also found on dendritic cells and rat macrophages but whether CD8 alpha enhances responses of a partner receptor like TCR to activate these cells is not known
STEMCELL Technologies EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit, For processing: 20 mL biofluid processed
The EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9 and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.
Biotium CD62L (L-Selectin)(CD62L/1588), Biotin conjugate, 0.1mg/mL
Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecti
Biotium CD62L (L-Selectin)(LAM1-116), CF488A conjugate, 0.1mg/mL
Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecti
Biotium CD62L (L-Selectin)(LAM1-116), Biotin conjugate, 0.1mg/mL
Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecti