Cell Line Lysates
Sino Biological Recombinant Human PPM1H Protein (full length, GST Tag), Active 10
Recombinant full length human PPM1H was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Rockland Immunochemicals U-251 Whole Cell Lysate 500µg
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µL depending on the size format of your gel.
INTACT GENOMICS INC BL21(DE3) Electrocompetent Cells 12x100µl
Intact Genomics BL21 (DE3) electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21(DE3) electrocompetent E. coli cells ideal for construction of complex expression libraries.BL21(DE3) Electrocompetent Cells have the following features: >1 x 10^10 cfu/µg efficiency with electroporation Widely used host background T7 Expression Strain Deficient in both lon (1) and ompT proteases Resistant to phage T1 (fhuA2) B StrainProduct Includes and Storage:BL21(DE3) Electrocompetent Cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4 ºC
Sino Biological Recombinant Human CNR1 Protein (Nanodisc, Flag-His Tag)
A DNA sequence encoding the Human CNR1 (P21554-1)(Asp74-Ala305) & (Asp333-Ser414) was expressed and linked by a Fusion Protein, with a Flag tag followed by a polyhistidine tag at the N-terminus. Within the expressed regions, there are several proprietary thermostabilizing mutations.