Cell Culture, Stem Cell Culture
Chromatin Immunoprecipitation, Western Blot
ELISA, Western Blot
Immunoprecipitation, In vitro Assay, Western Blot
Immunoprecipitation, SDS-Page, Western Blot
Immunoprecipitation, Western Blot
In vitro Assay, Western Blot
Murine L cells were stably transfected with human CD40L (L6) and freeze-dried through proprietary process. These cells are primarily intended to activate human cells through triggering of CD40 surface antigen but were also demonstrated to activate B cells from several animal species. L6 cells successfully support the differentiation of human monocyte towards the dendritic cell lineage. L6 cells are included in the Human Blood B booster and the Animal Blood B Booster kits, and support B cell growth and Ig secretion, but can also used alone for transient cultures.
Protein Extraction, Protein Preparation
SDS-Page, Western Blot
These Lysates Have Not Been Subjected to Denaturing or Reducing Conditions. This Allows The Tissue or Cell Lysate to be Used in a Variety of Applications; to Study Protein-Protein Interaction, Ligand Binding, ELISA, Immunoprecipitation, 1 D and 2 D Gel Electrophoresis, and Western Blotting for The Detection of Specific Protein Targets. For Use in 1 D and 2 D Gel Electrophoresis, The Addition of a Denaturing Gel Loading Buffer with Reducing Agents May be Required. Buffer Requirements for Performing Protein-Protein Interaction and Ligand Binding Studies Can Vary Significantly From RIPA Buffer and May Require Modifications. In Most Cases, Tissue Lysates in RIPA Buffer Can be Used, Directly in Standard ELISA and Immunoprecipitation Assays. These Lysates Are Proteomic Discovery Tools. Researchers Should Validate and Optimize for Individual Use.
This Calpain 5 antibody is intended for use as a positive control in Western Blot. You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
This Product is Intended for Use as a Positive Control in Western Blot. You Will Receive 1 Vial of Lysate (100 ug), 1 Vial of Empty Vector Negative Control (100 ug), and 1 Vial of 2 x SDS Sample Buffer (250 uL).
This Product is Intended for Use as a Positive Control in Western Blot. You Will Receive 1 Vial of Lysate (100 ug), 1 Vial of Empty Vector Negative Control (100 ug), and 1 Vial of 2 xSDS Sample Buffer (250 ul).
This product is intended for use as a positive control in Western Blot. You will receive 1 vial of lysate (100 ug), 1 vial of empty vector negative control (100 ug), and 1 vial of 2X SDS sample buffer (250 uL).