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A DNA sequence encoding the Influenza A virus (A/District of Columbia/27/2023(H3N2)) Neuraminidase / NA (EPI2990330) (Ile30-Thr469) was expressed with a polyhistidine tag at the C-terminus.
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A DNA sequence encoding the Influenza A virus ((A/California/07/09) (H1N1)) hemagglutinin (translated amino acids of ACP41953.1), termed as HA, was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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A DNA sequence encoding the Influenza A virus (A/Massachusetts/18/2022; A/Croatia/10136RV/2023 (H3N2)) neuraminidase (translated amino acids of EPI2096147) (Ile30-Thr469) was expressed with a vasodilator-stimulated phosphoprotein tetramerization domain at the N-terminus and a polyhistidine tag at the C-terminus.
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