Intact Genomics (IG) i7 High-Fidelity DNA Polymerase 2x master mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7 high fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has high fidelity, sensitivity and processivity with an error rate ~2.8×102-fold lower than Taq DNA polymerase, and significantly lower than other proofreading enzymes in the marketplace (1).Applications Long and difficult template DNA amplification Cloning High-fidelity PCR Efficient for amplifying high GC content template DNA with magic enhancer.