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Filtered Search Results
Thermo Scientific™ BglI (10 U/μL)
Cut at GCCNNNN^NGGC sites with BglI restriction enzyme, which performs best at 37°C in O buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bgl I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ DraI (10 U/μL)
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.
Thermo Scientific™ Eco24I (BanII) (10 U/μL)
Cut at GRGCY^C sites with Eco24I (BanII) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BanII, EcoT38I, FriOI).
Thermo Scientific™ Eco47III (AfeI) (10 U/μL)
The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco47III (AfeI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Eam1104I (EarI) (10 U/μL)
The Eam1104I (EarI) restriction enzyme recognizes CTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bst6I, EarI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eam1104I (EarI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Scarless Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | Yes |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Eco52I (EagI) (10 U/μL)
The Eco52I (EagI) restriction enzyme recognizes C^GGCCG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BseX3I, BstZI, EagI, EclXI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco52I (EagI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer Eco52I (EagI)) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ PstI (10 U/μL)
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ BspTI (AflII) (10 U/μL)
Cut at C^TTAAG sites with BspTI (AflII) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I).
Thermo Scientific™ BseLI (BslI) (10 U/μL)
Cut at CCNNNNN^NNGG sites with BseLI (BslI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: AfiI, Bsc4I, BslI).
Thermo Scientific™ BplI (5 U/μL)
Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | BplI |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ Bpu10I (5 U/μL)
Cut at CCTNAGC(-5/-2)^ sites with Bpu10I restriction enzyme, which performs best at 37°C in its own unique buffer.
Thermo Scientific™ SdaI (SbfI) (10 U/μL)
The SdaI (SbfI) restriction enzyme recognizes CCTGCA^GG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: SbfI, Sse8387I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SdaI (SbfI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer SdaI (SbfI)) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ SmiI (SwaI) (10 U/μL)
Cut at ATTT^AAAT sites with SmiI (SwaI) restriction enzyme, which performs best at 30°C in O buffer (Isoschizomers: SwaI).
Thermo Scientific™ HpyF3I (DdeI) (10 U/μL)
The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | HpyF3I (DdeI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ TauI (3 U/μL)
The TauI restriction enzyme recognizes GCSG^C sites and cuts best at 55°C in B buffer.
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | TauI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer B |
| Type IIS RE | No |
| Optimal Reaction Temperature | 55°C |
| Concentration | 3 U/μL |