Restriction Enzymes

Restriction Enzymes
- (1)
- (65)
- (597)
- (2)
- (1)
- (2)
- (1)
- (1)
- (4)
- (2)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (4)
- (2)
- (1)
- (2)
- (2)
- (4)
- (9)
- (1)
- (5)
- (2)
- (1)
- (3)
- (2)
- (1)
- (2)
- (1)
- (1)
- (4)
- (5)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (2)
- (3)
- (2)
- (1)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (1)
- (3)
- (2)
- (2)
- (1)
- (1)
- (1)
- (3)
- (2)
- (1)
- (2)
- (1)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (1)
- (3)
- (3)
- (3)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (3)
- (2)
- (1)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (7)
- (4)
- (2)
- (2)
- (1)
- (1)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (3)
- (2)
- (3)
- (4)
- (2)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (2)
- (1)
- (1)
- (1)
- (2)
- (1)
- (1)
- (8)
- (1)
- (1)
- (3)
- (1)
- (3)
- (2)
- (1)
- (1)
- (1)
- (3)
- (1)
- (2)
- (1)
- (2)
- (3)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (4)
- (9)
- (1)
- (1)
- (5)
- (3)
- (1)
- (2)
- (2)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (2)
- (6)
- (2)
- (2)
- (2)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (2)
- (1)
- (4)
- (4)
- (2)
- (2)
- (4)
- (3)
- (2)
- (2)
- (1)
- (1)
- (2)
- (2)
- (3)
- (2)
- (3)
- (2)
- (1)
- (2)
- (1)
- (1)
- (1)
- (1)
- (7)
- (8)
- (7)
- (1)
- (1)
- (11)
- (1)
- (1)
- (1)
- (1)
- (2)
- (1)
- (3)
- (2)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (2)
- (6)
- (1)
- (1)
- (2)
- (1)
- (4)
- (5)
- (2)
- (4)
- (2)
- (2)
- (2)
- (5)
- (6)
- (2)
- (1)
- (2)
- (4)
- (1)
- (1)
- (3)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (1)
- (3)
- (2)
- (7)
- (1)
- (1)
- (1)
- (1)
- (1)
- (3)
- (1)
- (1)
- (2)
- (1)
- (2)
- (3)
- (1)
- (2)
- (2)
- (3)
- (1)
- (3)
- (1)
- (1)
- (2)
- (2)
- (2)
- (2)
- (1)
- (1)
- (2)
- (1)
- (8)
- (1)
- (2)
- (9)
- (2)
- (2)
- (2)
- (2)
- (562)
- (81)
- (90)
- (551)
- (10)
- (574)
- (2)
- (24)
- (28)
Filtered Search Results

Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | EcoR I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ EcoRI (10 U/μL)

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.

Concentration | 10 U/μL |
---|
Thermo Scientific™ EcoRII (10 U/μL)
Cut at ^CCWGG sites with EcoRII restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).

Thermo Scientific™ FastDigest SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | SacI |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 3' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | BamH I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest HindIII

The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Hind III |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest SalI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ BamHI (10 U/μL)
The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer.

Concentration | 10 U/μL |
---|
Thermo Scientific™ EcoRI, HC (50 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ ATP Solution (100 mM)

Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nco I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest Value Pack
FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in a single buffer.
Thermo Scientific™ FastDigest KpnI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest ScaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
