Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ FastDigest Bsp1407I
Cut at T^GTACA sites with Bsp1407I restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: BsrGI, BstAUI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bsp1407I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 80°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest XagI
Cut at CCTNN^NNNAGG sites with Thermo Scientific™ FastDigest™ EcoNI (XagI), which performs best using universal FastDigest Buffer (Isoschizomers: BstENI).
Thermo Scientific™ LguI (SapI) (5 U/μL)
The LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: BspQI, PciSI, SapI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | LguI (SapI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Scarless Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | Yes |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ FastDigest Bpu1102I
Cut at GC^TNAGC sites with Thermo Scientific™ FastDigest™ BlpI (Bpu1102I), which performs best using universal FastDigest Buffer (Isoschizomers: Bsp1720I, CelII).
Thermo Scientific™ Eco72I (PmlI) (10 U/μL)
Cut at CAC^GTG sites with Eco72I (PmlI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AcvI, BbrPI, PmaCI, PmlI, PspCI).
Thermo Scientific™ FastDigest LguI (IIs class)
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest Tru1I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ PacI (10 U/μL)
The PacI restriction enzyme recognizes TTAAT^TAA sites and cuts best at 37°C in its own unique buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PacI |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer PacI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Pfl23II (BsiWI) (3 U/μL)
Cut at C^GTACG sites with Pfl23II (BsiWI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsiWI, PspLI).
Thermo Scientific™ FastDigest Eam1105I
Cut at GACNNN^NNGTC sites with Thermo Scientific™ FastDigest™ Eam1105I, which performs best using universal FastDigest Buffer (Isoschizomers: AhdI, AspEI, BmeRI, DriI, EclHKI).
Thermo Scientific™ FastDigest PdiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ XapI (ApoI) (10 U/μL)
Cut at R^AATTY sites with XapI (ApoI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AcsI, ApoI).
Thermo Scientific™ BclI (10 U/μL)
Cut at T^GATCA sites with BclI restriction enzyme, which performs best at 55°C in G buffer (Isoschizomers: FbaI, Ksp22I).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ Bsp119I (BstBI) (10 U/μL)
Cut at TT^CGAA sites with Bsp119I (BstBI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuIm).
Thermo Scientific™ FastDigest MreI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.