Restriction Enzymes
Thermo Scientific™ SspDI (KasI) (10 U/μL)
Cut at G^GCGCC sites with SspDI (KasI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Thermo Scientific™ FastDigest EheI
Cut at GGC^GCC sites with Thermo Scientific™ FastDigest™ EheI, which performs best using universal FastDigest Buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
Thermo Scientific™ AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X)
Optimize digestions with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes using this buffer.
Thermo Scientific™ AluI (10 U/μL)
Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).
Thermo Scientific™ FastDigest Eco47III
Cut at AGC^GCT sites with Thermo Scientific™ FastDigest™ AfeI (Eco47III), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest Bst1107I
Cut at GTA^TAC sites with Thermo Scientific™ FastDigest™ BstZ17I (Bst1107I), which performs best using universal FastDigest Buffer (Isoschizomers: BssNAI).
Thermo Scientific™ Cfr42I (SacII) (10 U/μL)
The Cfr42I (SacII) restriction enzyme recognizes CCGC^GG sites and cuts best at 37°C in B buffer (Isoschizomers: KspI, SacII, Sfr303I, SgrBI, SstII).
Thermo Scientific™ ApaI (10 U/μL)
Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).
Thermo Scientific™ BglI (10 U/μL)
Cut at GCCNNNN^NGGC sites with BglI restriction enzyme, which performs best at 37°C in O buffer.
Thermo Scientific™ Van91I (PflMI) (10 U/μL)
Cut at CCANNNN^NTGG sites with Van91I (PflMI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccB7I, BasI, PflMI).
Thermo Scientific™ EcoRII (10 U/μL)
Cut at ^CCWGG sites with EcoRII restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).
Invitrogen™ Anza™ 93 HpaII
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ XhoI (10 U/μL)
Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).
Thermo Scientific™ MlsI (MscI) (5 U/μL)
The MlsI (MscI) restriction enzyme recognizes TGG^CCA sites and cuts best at 37°C in R buffer (Isoschizomers: BalI, MluNI, MscI, Msp20I).
Thermo Scientific™ FastDigest FaqI
Cut at GGGAC(10/14)^ sites with Thermo Scientific™ FastDigest™ BsmFI (FaqI), which performs best using universal FastDigest Buffer (Isoschizomers: BslFI).