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Filtered Search Results

Thermo Scientific™ AarI (2 U/μL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | AarI |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | Unique Buffer (10X Buffer AarI) |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 2 U/μL |
Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Nco I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ DpnI (10 U/μL)

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).

Concentration | 10 U/μL |
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Thermo Scientific™ PvuII (10 U/μL)
The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.

Concentration | 10 U/μL |
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Thermo Scientific™ FastDigest BcuI

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).

Sensitive to Heat Inactivation | No |
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Product Type | Restriction Enzyme |
Enzyme | Bcu I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Product Line | FastDigest™ |
Thermo Scientific™ Tru1I (MseI) (10 U/μL)

Cut at T^TAA sites with Tru1I (MseI) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: MseI, Tru9I).

Sensitive to Heat Inactivation | No |
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Product Type | Restriction Enzyme |
Enzyme | Tru1I (MseI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer R |
Type IIS RE | No |
Optimal Reaction Temperature | 65°C |
Concentration | 10 U/μL |
Thermo Scientific™ FastDigest Bpu1102I

Cut at GC^TNAGC sites with Thermo Scientific™ FastDigest™ BlpI (Bpu1102I), which performs best using universal FastDigest Buffer (Isoschizomers: Bsp1720I, CelII).

Thermo Scientific™ FastDigest Eco31I (IIs class)

The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Eco31I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest AatII

Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).

Thermo Scientific™ FastDigest MssI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest MspI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest Eco91I

Cut at G^GTNACC sites with Thermo Scientific™ FastDigest™ Eco91I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Thermo Scientific™ KpnI (10 U/μL)
The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Kpn I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | Unique Buffer (10X Buffer KpnI) |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ FastDigest BglII

Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | No |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Bgl II |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Product Line | FastDigest™ |
Thermo Scientific™ HindIII (10 U/μL)
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

Concentration | 10 U/μL |
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