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Filtered Search Results
Thermo Scientific™ KpnI (10 U/μL)
The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Kpn I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer KpnI) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest VspI
Cut at AT^TAAT sites with Thermo Scientific™ FastDigest™ AseI (VspI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PshBI).
Thermo Scientific™ HpaII (10 U/μL)
The HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Hpa II |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ MluI (10 U/μL)
The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Mlu I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest BclI
Cut at T^GATCA sites with Thermo Scientific™ FastDigest™ BclI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FbaI, Ksp22I).
Thermo Scientific™ FastDigest AluI
Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI).
Thermo Scientific™ FastDigest PdmI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Invitrogen™ Anza™ 3 BcuI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Sensitive to Heat Inactivation | No |
| Product Type | Restriction Enzyme Cloning System |
| Enzyme | Bcu I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Anza™ 10X Red Buffer, Anza™ 10X Buffer |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | Anza |
Thermo Scientific™ Bsp68I (NruI) (10 U/μL)
Cut at TCG^CGA sites with Bsp68I (NruI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BtuMI, NruI).
Thermo Scientific™ Bsp119I (BstBI) (10 U/μL)
Cut at TT^CGAA sites with Bsp119I (BstBI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuIm).
Thermo Scientific™ Eco91I (BstEII) (10 U/μL)
The Eco91I (BstEII) restriction enzyme recognizes G^GTNACC sites and cuts best at 37°C in O buffer (Isoschizomers: BstEII, BstPI, EcoO65I, PspEI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco91I (BstEII) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ PauI (BssHII) (10 U/μL)
The PauI (BssHII) restriction enzyme recognizes G^CGCGC sites and cuts best at 37°C in R buffer (Isoschizomers: BsePI, BssHII).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PauI (BssHII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest Eco81I
Cut at CC^TNAGG sites with Thermo Scientific™ FastDigest™ Bsu36I (Eco81I), which performs best using universal FastDigest Buffer (Isoschizomers: AxyI, Bse21I).
Thermo Scientific™ AloI (2 U/μL)
Cut at ^(7/12-13)GAAC(N)6TCC(12-13/7)^ sites with AloI restriction enzyme, which performs best at 30°C in R buffer.
Thermo Scientific™ FastDigest Bsu15I
Cut at AT^CGAT sites with ClaI (Bsu15I) restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bsu15I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |