Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ PdmI (XmnI) (10 U/μL)
The PdmI (XmnI) restriction enzyme recognizes GAANN^NNTTC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Asp700I, MroXI, XmnI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PdmI (XmnI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ SspI (10 U/μL)
The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Ssp I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer G |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest AluI
Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI).
Thermo Scientific™ PauI (BssHII) (10 U/μL)
The PauI (BssHII) restriction enzyme recognizes G^CGCGC sites and cuts best at 37°C in R buffer (Isoschizomers: BsePI, BssHII).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | PauI (BssHII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest LguI (IIs class)
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Eco72I (PmlI) (10 U/μL)
Cut at CAC^GTG sites with Eco72I (PmlI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AcvI, BbrPI, PmaCI, PmlI, PspCI).
Thermo Scientific™ FspAI (5 U/μL)
The FspAI restriction enzyme recognizes RTGC^GCAY sites and cuts best at 37°C in O buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | FspAI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ FastDigest Bsu15I
Cut at AT^CGAT sites with ClaI (Bsu15I) restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bsu15I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ FastDigest SaqAI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest Bsp120I
Cut at G^GGCCC sites with Thermo Scientific™ FastDigest™ Bsp120I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspOMI).
Thermo Scientific™ FastDigest Eco81I
Cut at CC^TNAGG sites with Thermo Scientific™ FastDigest™ Bsu36I (Eco81I), which performs best using universal FastDigest Buffer (Isoschizomers: AxyI, Bse21I).
Thermo Scientific™ TaiI (MaeII) (10 U/μL)
Cut at ACGT^ sites with TaiI (MaeII) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: HpyCH4IV, MaeII).
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | TaiI (MaeII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 65°C |
| Concentration | 10 U/μL |
Thermo Scientific™ MauBI (5 U/μL)
Cut T CG^CGCGCG sites with MauBI restriction enzyme, which performs best at 37°C in Tango buffer.
Thermo Scientific™ Eco130I (StyI) (10 U/μL)
Cut at C^CWWGG sites with Eco130I (StyI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI).
Invitrogen™ Anza™ 42 RsaI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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