Restriction Enzymes
Thermo Scientific™ ScaI (10 U/μL)
The ScaI restriction enzyme recognizes AGT^ACT sites and cuts best at 37°C in its own unique buffer (Isoschizomers: AssI, BmcAI, ZrmI).
Thermo Scientific™ Bsu15I (ClaI) (10 U/μL)
Cut at AT^CGAT sites with Bsu15I (ClaI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI).
Thermo Scientific™ SmaI (10 U/μL)
Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).
Invitrogen™ Anza™ 93 HpaII
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ Eam1105I (AhdI) (10 U/μL)
Cut at GACNNN^NNGTC sites with Eam1105I (AhdI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: AhdI, AspEI, BmeRI, DriI, EclHKI).
Thermo Scientific™ MlsI (MscI) (5 U/μL)
The MlsI (MscI) restriction enzyme recognizes TGG^CCA sites and cuts best at 37°C in R buffer (Isoschizomers: BalI, MluNI, MscI, Msp20I).
Thermo Scientific™ BfmI (SfcI) (10 U/μL)
Cut at C^TRYAG sites with BfmI (SfcI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstSFI, SfcI).
Thermo Scientific™ ApaI (10 U/μL)
Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).
Thermo Scientific™ BshTI (AgeI) (10 U/μL)
Cut at A^CCGGT sites with BshTI (AgeI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AgeI, AsiGI, CspAI, PinAI).
Thermo Scientific™ FastDigest PstI
The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).
Thermo Scientific™ FastDigest BpiI (IIs class)
Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).
Thermo Scientific™ Eco105I (SnaBI) (10 U/μL)
The Eco105I (SnaBI) restriction enzyme recognizes TAC^GTA sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstSNI, SnaBI).
Thermo Scientific™ PaeI (SphI) (10 U/μL)
The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI).
Thermo Scientific™ FastDigest Ecl136II
Cut at GAG^CTC sites with Thermo Scientific™ FastDigest™ Ecl136II, which performs best using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ FastDigest BstXI
Cut at CCANNNNN^NTGG sites with Thermo Scientific™ FastDigest™ BstXI, which performs best at 55°C in 5-15 minutes using universal FastDigest Buffer.