Substrates
Enzo Life Sciences Z-Leu-Arg-Gly-Gly-AMC (5mg)
Fluorogenic substrate for ubiquitin C-terminal hydrolases (UCHs), including UCH-L3 and Isopeptidase T. Sequence is based on the C-terminal residues of ubiquitin. Ex.: 380 nm Em.: 460 nm. Alternative name: Z-LRGG-AMC, Ubiquitin C terminal hydrolase substrate (fluorogenic), UCH substrate (fluorogenic). Purity: ≥95%. Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences Z-LR-AMC (10mg)
Sensitive fluorogenic substrate for cathepsin K (Km=8µM, kcat/Km=4 x 10 M-1s-1). This substrate is also cleaved by cathepsin B, F, L, L2/V, S. Z-LR-AMC is also cleaved by malaria parasite cysteine proteases falcipain-1, -2, -3, berghepain, and vivapain-2, -3. Ex.: 365nm, Em.: 440nm, although the following Ex/Em can also be used: 355,380/450,460. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-Leu-Arg-AMC, Cathepsin K substrate (fluorogenic). Purity: ≥97% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO to at least 20mM. Long Term Storage: -20°C.
Sino Biological Peptide Substrates: Amyloid Beta Peptide (1-42)
The Amyloid Beta Peptide (42 aa) sequence is DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA. It is produced synthetically and treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) prior to drying which breaks down pre-formed fibrils and monomerizes the peptide.
Gold Biotechnology Inc D-Luciferin, Sodium Salt (Proven and Published™) 100 mg
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Abcam Carbo x yl-DCFDA (5(6)-Carbo x y-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate, 1G
MW 529.28 Da, Purity >85%. Amine-reactive, fluorogenic, non-selective esterase substrate. Hydrolyses to DFCA (ab145439) fluorophore intracellularly. Labels intact cells. 2,7-Dichlorofluorescein (ab146332) derivative and DCFDA N-succinimidyl ester (ab145286) precursor.
The product is subject to the following: Abcam Restricted Use Statement
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Proven and Published™) 2 x 1 g
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Cayman Chemical Cbz-Gly-Pro-AMC 5mg
A fluorogenic substrate for prolyl endopeptidase; AMC is released upon enzymatic cleavage by prolyl endopeptidase and its fluorescence can be used to quantify prolyl endopeptidase activity; ex/em = 340-360/440-460 nm, respectively
Medchemexpress LLC Recombinant rat HER2 (ErbB2) protein, HEK293-expressed, C-terminal His tag | >95.0% | 50 UG
Recombinant rat HER2 (ErbB2/CD340) protein expressed in HEK293 cells and supplied lyophilized with a C-terminal 10xHis tag. Apparent molecular weight is ~91 kDa, and purity is greater than 95% by reducing SDS-PAGE. Formulated from 20 mM PB, 150 mM NaCl, pH 7.4, it is intended for research applications such as binding assays, antibody validation, and functional studies. Store lyophilized at -20°C and follow reconstitution guidance.
Sino Biological Peptide Substrates: AKT (PKB) Substrate
The AKT (PKB) Substrate peptide sequence (CKRPRAASFAE) is based on the N-terminus of GSK3.
Sino Biological Peptide Substrates: Modified AKT Substrate II
The Modified AKT Substrate II peptide sequence (modified-CKRPRAASFAE) is based on the N-terminus of GSK3.
Enzo Life Sciences Ac-LEHD-AFC (10mg). CAS: 210345-03-2
Fluorogenic substrate for caspase-9. Alternative name: Caspase 9 substrate (fluorogenic). Formula: C33H38F3N7O11. MW: 765.7. Purity: ≥96% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO. Long Term Storage: -20°C.