Chromatography and Mass Spectrometry Reagents

Chromatography and Mass Spectrometry Reagents










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Thermo Scientific™ Pierce™ Dimethylformamide (DMF), Sequencing grade
Perform liquid chromatography (HPLC) and mass spectrometry (LC-MS) applications with this 99.5%-pure DMF solvent (MW 73.09, CAS 68-12-2) with low UV-absorptivity.
Thermo Scientific™ BSTFA + 1% TMCS Silylation Reagent

Ideal for difficult-to-silylate compounds, the Thermo Scientific™ BSTFA + 1% TMCS Silylation Reagent is derivatizing compounds.
Storage | 2°C to 8°C |
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Thermo Scientific™ Hydrocarbon Soluble Siliconizing Fluid
For siliconizing glass surfaces use Thermo Scientific™ Hydrocarbon Soluble Siliconizing Fluid.
Standard Components | 1,7-Dichloro-1,1,3,3,5,5,7,7-Octamethyltetrasiloxane |
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Thermo Scientific™ Perfluoroacylimidazoles (TFAI and HFBI) Acylation Reagents
Conduct acylation of hydroxyl groups and primary and secondary amines with the Thermo Scientific™ Perfluoroacylimidazoles (TFAI and HFBI).
For Use With | FID and ECD |
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Standard Concentration | 98 to 100% |
Thermo Scientific™ Methoxamine (MOX) Reagent

Prepare oximes of steroids and ketoacids prior to silylation with the Thermo Scientific™ Methoxamine (MOX) Reagent.
Thermo Scientific™ Pierce™ Heptafluorobutyric Acid (HFBA), Sequencing Grade
Bottles and ampules of 99.5%-pure HFBA (MW 214.04, CAS 375-22-4), valid for use as an ion-pairing agent in reverse-phase peptide separations (HPLC).
Thermo Scientific™ Pierce™ 0.1% Formic Acid (v/v) in Water, LC-MS Grade
An LC/MS-grade preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.
Item Name | Formic Acid |
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Thermo Scientific™ Pierce™ 0.1% Trifluoroacetic Acid (v/v) in Water, LC-MS Grade
An LC/MS-grade preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.
Thermo Scientific™ Pierce™ 0.1% Formic Acid (v/v) in Acetonitrile, LC-MS Grade
An LC/MS-grade preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.
Thermo Scientific™ Pierce™ Dimethylsulfoxide (DMSO), LC-MS Grade
A sequencing-grade preparation with high purity and consistent absorptivity properties, for use in HPLC and mass spectrometry methods.
Thermo Scientific™ Pierce™ Formic Acid, LC-MS Grade

A high-purity solvent supplied in glass ampules as a convenient, contamination-free alternative for preparing elution solvents for HPLC separations of protein and peptides.
Form | Liquid |
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Reagent Type | Solvent (Pure) |
Product Line | Pierce™ |
Thermo Scientific™ Pierce™ Acetonitrile (ACN), LC-MS Grade

An LC/MS-grade preparation with high purity and low UV-absorptivity that makes it suitable for HPLC and mass spectrometry applications.
For Use With | HPLC, Mass Spectrometry |
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Form | Liquid |
Reagent Type | Solvent (Pure) |
Thermo Scientific™ TMCS Silylation Reagent
Catalyze the derivatization of difficult-to-silylate compounds using Thermo Scientific™ TMCS Silylation Reagent.
Thermo Scientific™ Tri-Sil BP (BSA:Pyridine) Reagent

Employ a reagent-solvent mixture, for one-step derivatization with the Thermo Scientific™ Tri-Sil BP (BSA:Pyridine) Reagent,
Thermo Scientific™ MSTFA and MSTFA + 1% TMCS Silylation Reagent

Offer maximum volatility with the Thermo Scientific™ MSTFA and MSTFA + 1% TMCS Silylation Reagent.
For Use With | GC/MS |
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Thermo Scientific™ Tri-Sil TP (TMSI:Pyridine) Reagent
Derivatize hydroxyls compounds, particularly carbohydrates with Thermo Scientific™ Tri-Sil TP (TMSI:Pyridine) Reagent.
Standard Components | Pyridine, 1-(Trimethylsilyl)-1 H-imidazole |
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Storage | 2°C to 8°C |
For Use With | GC |
Standard Concentration | 65 - 80, 20 - 25 % |
Thermo Scientific™ MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent
Produce stable TBDMS derivatives for GC analysis with the Thermo Scientific™ MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent.
Storage | 2°C to 8°C |
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For Use With | GC |
Hazards | Irritant |
Thermo Scientific™ Tri-Sil HTP (HDMS:TMCS:Pyridine) Reagent
Employ a reagent-catalyst solvent mixture, for one-step derivatization with the Thermo Scientific™ Tri-Sil HTP (HDMS:TMCS:Pyridine) Reagent,
Standard Components | Pyridine, Hexamethyldisilazane, Chlorotrimethylsilane |
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Storage | 2°C to 8°C |
For Use With | GC |
Hazards | Corrosive, Flammable |
Standard Concentration | 65 - 80, 10 - 20, 5 - 7% |
Thermo Scientific™ MBTFA Acylation Reagent
Trifluoroacylate primary and secondary amines, hydroxyl and thiol groups, and carbohydrates with the Thermo Scientific™ MBTFA Acylation Reagent.
Standard Components | N-Methyl-bis(trifluoroacetamide) |
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Storage | -20°C |
For Use With | GC |
Hazards | Corrosive, Flammable |
Standard Concentration | 98 to 100% |
Acetonitrile with 0.1% Formic Acid (v/v), Optima™ LC/MS Grade, Thermo Scientific™

CAS: 75-05-8 Form: Liquid Grade: OPTIMA™ LC-MS Item Name: 0.1% Formic Acid in Acetonitrile (v/v) Reagent Type: Solvent Blends LCMS Gradient Suitability: Pass Test
Item Name | 0.1% Formic Acid in Acetonitrile (v/v) |
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CAS | 75-05-8 |
Form | Liquid |
Reagent Type | Solvent Blends |
Grade | OPTIMA™ LC-MS |
LCMS Gradient Suitability | Pass Test |
Thermo Scientific™ Pierce™ Dimethylsulfoxide (DMSO), Sequencing Grade
A sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods.
Thermo Scientific™ Methylate Reagent (DMFDMA)
For easy, effective preparation of methyl esters from fatty acids and amino acids use Thermo Scientific™ Methylate Reagent (DMFDMA).
Thermo Scientific™ Tri-Sil TBT (TMSI:BSA:TMCS) Reagent

Employ a catalyzed silylation reagent formulation, for derivatization of hydroxyls with the Thermo Scientific™ Tri-Sil TBT (TMSI:BSA:TMCS) Reagent.
Thermo Scientific™ MethElute™ Reagent (TMPAH)

For accurate, sensitive on-column methylation use Thermo Scientific™ MethElute™ Reagent (TMPAH).

Standard Components | Trimethylanilinium hydroxide in Methanol |
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Storage | Room Temperature |
For Use With | GC |
Hazards | Flammable, Toxic |
Standard Concentration | 0.2 M |
Thermo Scientific™ Pierce™ Heptafluorobutyric Acid (HFBA), HPLC Grade
Bottles and ampules of 99.5%-pure HFBA (MW 214.04, CAS 375-22-4), valid for use as an ion-pairing agent in reverse-phase peptide separations (HPLC).
Thermo Scientific™ Pentafluorobenzyl Bromide (PFBBr) Alkylation Reagent

Derivatize carboxyl acids, phenols, and sulfonamides for electron-capture detection (ECD) GC analysis with the Thermo Scientific™ Pentafluorobenzyl Bromide (PFBBr) Alkylation Reagent.
Thermo Scientific™ Perfluoro Acid Anhydrides (TFAA, PFAA and HFAA) Acylation Reagents

Prepare volatile and stable fluoracyl derivatives for GC/MS with the Thermo Scientific™ Perfluoro Acid Anhydrides (TFAA, PFAA and HFAA).
Storage | Room Temperature |
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For Use With | GC/MS |
Standard Concentration | 98 to 100% |
Thermo Scientific™ Pierce™ Triethylamine (TEA), Sequencing Grade
Bottles of 99.5%-pure TEA (MW 101.2, CAS 121-44-8) liquid (sold by weight); for HPLC use as an ion-pairing agent in reverse-phase peptide separations.
Thermo Scientific™ Silylation Grade Solvents

Use specially manufactured and packaged Thermo Scientific™ Silylation Grade Solvents to meet the exacting needs of silylation and other sensitive derivatization reactions.
Thermo Scientific™ Pierce™ Trifluoroacetic Acid (TFA), Sequencing grade
Bottles of 99.5%-pure TFA (MW 114.02, CAS 76-05-01) liquid (sold by weight); for HPLC use as an ion-pairing agent in reverse-phase peptide separations.
Form | Liquid |
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Formulation | 99.5%-Pure TFA (MW 114.02, CAS 76-05-01) |
Reagent Type | Ion Pairing Reagent |
Product Line | Pierce™ |