Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices SpectraDrop Micro-Volume Refills, 24-Well, Teflon-Coated, Pack of 3
Molecular Devices SpectraDrop Micro-Volume 24-well refill kit includes three low-volume, Teflon-coated bottom slides to replace worn slides in SpectraDrop micro-volume assemblies. Slides are designed for precise droplet placement and reproducible low-volume absorbance and quantitation using 2 μL or 4 μL configurations.
- Contains three low-volume 24-well bottom slides.
- Teflon-coated bottom slides for precise droplet placement.
- Compatible with micro-volume adapters and cover slides.
- Supports 2 μL and 4 μL low-volume configurations (system-level).
- Intended for micro-volume absorbance and sample quantitation.
Perkin Elmer US LLC Sample Capillary Adapter for GemCone and GemTip Nebulizers
This Capillary Adapter, or Gland Nut, is included in the GemTip High-Sensitivity Plastic Body Nebulizer Complete Assembly for AAnalyst (N0370393) and the Universal GemTip Corrosion-Resistant Plastic Body Nebulizer Complete Assembly for AAnalyst (N0370394). It is used in the GemCone Low Flow Nebulizer (N0770358) and the GemCone High Dissolved Solids Nebulizer (N0690670).
Molecular Devices Emission Filter, Time Resolved, 490 nm, 10 nm
Time-resolved emission filter designed for time-resolved fluorescence measurements. The optical filter centers at 490 nm with a 10 nm full width bandwidth and is supplied with a holder for insertion into compatible multi-mode microplate readers. Typical applications include donor emission detection in TR-FRET and other time-resolved assays.
- 490 nm center wavelength, 10 nm bandwidth.
- Includes holder for filter-slide insertion.
- Designed for time-resolved fluorescence applications.
- Optimized for donor emission detection in TR-FRET assays.
- Fits standard filter slots on compatible microplate readers.
THERMO SCI LSG 3P Universal Tray including 96- 384- and 6-48-well plate adapters for plate without lid
Universal Tray including 96- 384- and 6-48-well plate adapters for plate without lid
Revvity Health Sciences Inc HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
HTRF Human IL-2 High Performance Detection Kit, 10,000 Assay Points
Molecular Devices Absorbance Filter, 450 nm, 8 nm, FilterMax Compatible
Replaceable narrow-band absorbance (photometric) filter designed for use in FilterMax multimode microplate readers. The filter is centered at 450 nm and has an approximate 8 nm bandwidth, providing a specific narrow bandpass for fixed-wavelength absorbance measurements in microplates.
- Center wavelength: 450 nm.
- Bandwidth: 8 nm (approximate).
- Designed for fixed-wavelength absorbance assays.
- Installs in the instrument filter wheel for modular wavelength selection.
- Narrow-bandpass optical filter for consistent photometric readings.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Raf1 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total Raf1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total SMARCA-2 Detection Kit, 50,000 Assay Points
HTRF Human Total SMARCA-2 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL-12/IL-12Rβ1 Binding Kit, 500 Assay Points
HTRF Human IL-12/IL-12Rβ1 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Mouse Total STING Detection Kit, Control Lysate
HTRF Mouse Total STING Detection Kit, Control Lysate
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-ERα (Ser167) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-ERα (Ser167) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Total MSH3 Detection Kit, Control Lysate
HTRF Total MSH3 Detection Kit, Control Lysate
Molecular Devices Absorbance Filter, 260 nm, 5.5 nm Bandwidth
The Molecular Devices 260 nm absorbance filter (5.5 nm bandwidth) is an optical bandpass accessory designed for use with compatible FilterMax series multimode microplate readers. It provides a narrow UV transmission centered at 260 nm for precise absorbance measurements of nucleic acids and other UV-absorbing analytes.
- Provides narrow bandpass transmission centered at 260 nm.
- 5.5 nm bandwidth for selective UV absorbance measurements.
- Suitable for nucleic acid quantification and other UV-absorbing samples.
- Designed for easy installation in modular filter holders.
- Improves measurement specificity by reducing out-of-band light.
Molecular Devices Fluorescence Emission Filter, 660 nm, 32 nm Bandwidth
This emission filter is centered at 660 nm with a 32 nm full bandwidth and is intended for use in filter-based fluorescence microplate readers to isolate red emission wavelengths for assays using long-wavelength fluorophores.
- Center wavelength: 660 nm.
- Bandwidth (full width at half maximum): 32 nm.
- Product type: fluorescence emission filter for microplate readers.
- Application: isolates red emission for assays using red fluorophores and dyes.
- Benefit: improves spectral selectivity and reduces signal crosstalk between channels.