Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc AlphaScreen Rabbit IgG Detection Kit, 10,000 Assay Points
AlphaScreen Rabbit IgG Detection Kit, 10,000 Assay Points
Electron Microscopy Sciences NIGHTSEA™ Fluorescence Adapter Barrier Filter, Ultra Violet
Additional combinations excitation/ emission wavelength combination can be added NIGHTSEA™ Stereo Microscope Fluorescence Viewing system by purchasing individual light head, barrier filter, and viewing shield combinations. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.
Color: Ultra Violet
Excitation: 360-380nm
Emission filter: 415 nm longpass
Fluorophores: DAPI
Revvity Health Sciences Inc HTRF Human and Mouse Total RNA Pol II Detection Kit, Control Lysate
HTRF Human and Mouse Total RNA Pol II Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total HSP27 Detection Kit, 10,000 Assay Points
HTRF Human Total HSP27 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Histone H3 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total Histone H3 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total H2AX Detection Kit, Control Lysate
HTRF Human Total H2AX Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total CDK12 Detection Kit, 10,000 Assay Points
HTRF Human Total CDK12 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF PROTAC Binding Buffer #1, 200 mL
HTRF PROTAC Binding Buffer #1, 200 mL
Revvity Health Sciences Inc HTRF Human Phospho-MerTK (Tyr749) Detection Kit, Control Lysate
HTRF Human Phospho-MerTK (Tyr749) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse TOM20 Detection Kit Standard
HTRF Human & Mouse TOM20 Detection Kit Standard
Molecular Devices Emission Filter, 650 nm, 40 nm Bandwidth, Fluorescence
Molecular Devices fluorescence emission bandpass filter with a center wavelength of 650 nm and a 40 nm full bandwidth, designed for spectral selection of red and far-red fluorophores in multi-mode plate readers and imaging systems.
- Provides 650 nm center wavelength with 40 nm full bandwidth.
- Optimized for fluorescence, luminescence, and TRF assays.
- Replaceable emission filter for compatible multi-mode microplate readers.
- Reduces background signal for selective detection of red/far-red fluorophores.
Molecular Devices Fluorescence Polarization Filter, 625 nm, 35 nm, Vertical
Fluorescence polarization emission filter with holder designed for use in compatible SpectraMax microplate readers. The filter provides a 625 nm center wavelength with a 35 nm bandwidth and a vertical polarization axis, optimized for fluorescence polarization assays and red-emitting fluorophores.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Polarized vertical orientation.
- Includes holder for slide mounting.
- Optimized for fluorescence polarization assays and red-emitting fluorophores.
- Compatible with microplate readers that accept filter slides.
Molecular Devices Fluorescence Filter Kit, EM 660 nm, 40 nm
The SpectraMax Fluorescence Intensity Filter Kit delivers five exchangeable optical filters for fluorescence, luminescence, and time-resolved fluorescence (TRF) assays on compatible multi-mode microplate readers. The kit includes an emission 660 nm filter with a 40 nm bandwidth plus additional excitation and emission filters to support common assays.
- Includes five exchangeable filters covering common fluorescence wavelengths from ~400-700 nm.
- Contains an emission 660 nm filter with a 40 nm full bandwidth for near-IR fluorescence detection.
- Supports fluorescence intensity, luminescence, and time-resolved fluorescence assay modes.
- Allows quick filter exchange to adapt to different assay requirements.
- Designed for use with compatible multi-mode microplate readers to expand detection flexibility.
Revvity Health Sciences Inc HTRF Human Total PLK1 Detection Kit, 500 Assay Points
HTRF Human Total PLK1 Detection Kit, 500 Assay Points