Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK5 Detection Kit, Control Lysate
HTRF Human & Mouse Total CDK5 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Phospho-c-RAF (Ser43) Detection Kit, Control Lysate
HTRF Phospho-c-RAF (Ser43) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-RAB10 (Thr73) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-RAB10 (Thr73) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total HPK1 Detection Kit, Control Lysate
HTRF Human Total HPK1 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF MDM2 Binding Kit, 10,000 Assay Points
HTRF MDM2 Binding Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC4 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total HDAC4 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc Tag-lite CLIP-Green Labeling Reagent, 20 nmoles
Tag-lite CLIP-Green Labeling Reagent, 20 nmoles
Research Products International Corp 0.5 ml Tube Adapter System for use with Micro-Tube Vortexer
Optional part for use with Micro-Tube Vortexer.
Revvity Health Sciences Inc HTRF Human & Mouse TOM20 Detection Kit Standard
HTRF Human & Mouse TOM20 Detection Kit Standard
Revvity Health Sciences Inc HTRF Human IL12/23 p40 Detection Kit, Standard, 1 Vial
HTRF Human IL12/23 p40 Detection Kit, Standard, 1 Vial
Revvity Health Sciences Inc HTRF Human Total BRD3 Detection Kit, 500 Assay Points
HTRF Human Total BRD3 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF AAV1 Detection Kit, 10,000 Assay Points
HTRF AAV1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF AAV5 Detection Kit, 10,000 Assay Points
HTRF AAV5 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total Cyclin A2 Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total Cyclin A2 Detection Kit, 100 Assay Points
Molecular Devices Validation Plate, Luminescence, Microplate, Recertifiable
The SpectraTest LM1 Luminescence Validation Plate from Molecular Devices provides a standardized reference for verifying luminescence performance of microplate readers. It enables routine performance checks and supports annual recertification to maintain calibration.
- Enables background noise and spike testing.
- Measures crosstalk between adjacent wells.
- Detects left-to-right positional bias across the plate.
- Supports annual recertification and calibration by an accredited laboratory.
- Intended for verification of luminescence detection performance in microplate readers.
