Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices Emission Filter, 650 nm, 40 nm, FilterMax
Replacement fluorescence emission filter with a 650 nm center wavelength and 40 nm bandwidth, designed for use in Molecular Devices FilterMax F-series multi-mode microplate readers to collect far-red fluorescence emission.
- Center wavelength 650 nm.
- Bandwidth 40 nm for selective far-red detection.
- Designed for installation in filter slide assemblies.
- Enables assays requiring far-red emission collection with reduced background.
- Replacement part for multi-mode microplate reader fluorescence modules.
Ace Glass, Inc. 600ML VESSEL 600ML VESSEL
600mL vessel with a 48mm O.D. neck to fit a #50 Ace-Thred, for connection to an umbrella manifold. Vessel has sloped sides. Working capacity of vessel is about 1/2 of volume.
Electron Microscopy Sciences MultiLap™ Fish Otolith Polishing Tool 6-Position Alignment Fixture
For use with Model 195 - 6 Position MultiLap (50168-95).
Molecular Devices Fluorescence Polarization Filter, 635 nm, 35 nm, Vertical
Molecular Devices fluorescence polarization filter with holder designed for fluorescence polarization assays and FP-compatible detection on compatible multi-mode microplate readers. The filter is specified for a 635 nm center wavelength with a 35 nm bandwidth and is polarized to enable fluorescence polarization measurements.
- Designed for fluorescence polarization assays and FP detection modes.
- Center wavelength 635 nm with 35 nm bandwidth.
- Polarized to enable measurement of fluorescence anisotropy.
- Includes holder for installation into compatible filter assemblies.
- Intended as an accessory for multi-mode microplate readers that accept replaceable filters.
Molecular Devices Fluorescence Filter, 447 nm, 60 nm, With Holder
Fluorescence donor emission filter for SpectraMax iD5 and iD5e multi-mode microplate readers, centered at 447 nm with a 60 nm bandwidth, supplied with a holder for mounting and optical alignment.
- Isolates emission centered at 447 nm with a 60 nm bandwidth.
- Includes holder for secure mounting in the instrument filter module.
- Compatible with fluorescence and NanoBRET assays on multi-mode microplate readers.
- Improves signal separation for donor emission detection and assay sensitivity.
- Designed for easy installation and reliable optical alignment.