Microplate Readers and Reader Accessories

Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.

The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.

In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.

Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.

Features may include:

Monochromator- or filter-based wavelength selection
Visible only or UV/Vis spectrum, fluorescence, and luminescence
Multiple photomultiplier tubes (PMTs) for simultaneous assays
Temperature and CO₂/O₂controls
Modular design for adding functionality

Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.

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466

Revvity Health Sciences Inc HTRF Human Amyloid Î² 1-42 Detection Kit, 10,000 Assay Points

HTRF Human Amyloid Î² 1-42 Detection Kit, 10,000 Assay Points

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467

Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points

HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points

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468

Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit, 10,000 Assay Points

HTRF Human XIAP / BIR3 Binding Kit, 10,000 Assay Points

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469

Revvity Health Sciences Inc HTRF Human & Mouse Total p300 Detection Kit, 10,000 Assay Points

HTRF Human & Mouse Total p300 Detection Kit, 10,000 Assay Points

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470

Revvity Health Sciences Inc HTRF Human Total IDO1 Detection Kit, 10,000 Assay Points

HTRF Human Total IDO1 Detection Kit, 10,000 Assay Points

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471

Revvity Health Sciences Inc HTRF Human Total SMARCA-4 Detection Kit, 500 Assay Points

HTRF Human Total SMARCA-4 Detection Kit, 500 Assay Points

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472

Molecular Devices Excitation Filter, 540 nm, 25 nm Bandwidth, Bandpass

Molecular Devices FilterMax fluorescence excitation bandpass filter centered at 540 nm with a 25 nm full width, designed for targeted excitation in fluorescence microplate assays. It reduces out-of-band light to improve assay signal-to-noise and installs into compatible filter slide slots for rapid configuration.