Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
Molecular Devices Filter Lens, 560 nm, EMax and VMax Compatible
The 560 nm filter lens is an optical replacement filter designed for use with EMax and VMax microplate readers. It provides centered transmission at 560 nanometers for measurements that require that wavelength and is supplied as an individual replacement lens.
- Optical center wavelength 560 nm.
- Compatible with EMax and VMax microplate readers.
- Sold as a single replacement filter lens.
- Intended for fluorescence and absorbance applications requiring a 560 nm bandpass.
Revvity Health Sciences Inc HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, Control Lysate
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Phospho-MerTK (Tyr749) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-MerTK (Tyr749) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Ataxin 2 Detection Kit, 100,000 Assay Points
HTRF Human & Mouse Ataxin 2 Detection Kit, 100,000 Assay Points
Molecular Devices Luminescence Filter, 410 nm, BW 80 nm
This Molecular Devices luminescence emission filter (with holder) provides a 410 nm center wavelength with an 80 nm bandwidth for luminescence detection in microplate assays. It is listed as an accessory for SpectraMax iD series multi-mode microplate readers and is commonly used as a donor emission filter in assays such as BRET2.
- Provides a 410 nm center wavelength with an 80 nm bandwidth
- Includes holder for secure installation in filter slot
- Optimized for luminescence emission detection
- Used as donor emission filter in luminescence assays
- Compatible with multi-mode microplate reader filter systems
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC5 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total HDAC5 Detection Kit, 500 Assay Points
Molecular Devices Absorbance Filter, 280 nm, 5.5 nm Bandwidth
Molecular Devices 280 nm absorbance filter designed as a replacement slide-style optical filter for select multimode microplate readers. The narrow 5.5 nm bandwidth enables precise absorbance measurements at 280 nm for protein quantitation and other absorbance-based assays.
- Provides narrowband absorbance detection at 280 nm with a 5.5 nm bandwidth.
- Optimized for protein quantitation and small-volume absorbance assays.
- Slide-style form factor suitable for insertion into accessory filter slots.
- Low stray light and high transmission within the passband.
- Intended as a replacement optical filter to restore instrument performance.
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 100,000 Assay Points