Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Molecular Devices Fluorescence Excitation Filter, 590 nm, 20 nm Bandwidth
Replacement fluorescence excitation filter for Molecular Devices FilterMax and compatible multi-mode microplate readers. Provides excitation centered at 590 nm for fluorescence assays and is sold as a replacement filter slide for fluorescence detection.
- Excitation center wavelength: 590 nm.
- Bandwidth: 20 nm.
- Intended for microplate fluorescence detection.
- Fits standard filter slide holders in compatible readers.
Molecular Devices Fluorescence Filter, Holder, 360 nm, BW 35 nm
Fluorescence filter with holder for installation in compatible microplate readers. Provides excitation in the near-UV at a 360 nm center wavelength with a 35 nm bandwidth; supplied with a holder for secure mounting and intended for fluorescence-based microplate assays.
- Provides 360 nm center wavelength excitation.
- 35 nm bandwidth for selective spectral isolation.
- Supplied with holder for microplate reader installation.
- Designed for fluorescence detection in microplate assays.
- Suitable for assays requiring near-UV excitation and narrow-band filtering.
Molecular Devices Filter Lens, 630 nm, Emission
The 630 nm filter lens is an interchangeable emission filter designed to enable red-region fluorescence detection on compatible microplate readers. It provides selective transmission centered at 630 nm to improve signal specificity for assays using red-emitting fluorophores.
- Center wavelength 630 nm.
- Designed for emission detection in the red spectral region.
- Interchangeable filter format for compatible microplate readers.
- Enhances signal specificity and reduces background for fluorescence assays.
- Single-unit form factor suitable for routine assay configuration changes.
Molecular Devices Fluorescence Polarization Filters, 535 nm, Polarized
Molecular Devices fluorescence polarization filter set designed for fluorescence polarization (FP) assays on compatible SpectraMax and FlexStation multi-mode microplate readers. The set contains polarized filters in vertical and horizontal orientations centered at 535 nm with a 25 nm bandwidth to support accurate FP measurements.
- Enables fluorescence polarization measurements.
- Includes vertical and horizontal polarized filters.
- Center wavelength 535 nm, bandwidth 25 nm.
- Includes two filters for polarization detection.
- Designed for compatible multi-mode microplate readers.
Molecular Devices Filter Lens, 560 nm, EMax and VMax Compatible
The 560 nm filter lens is an optical replacement filter designed for use with EMax and VMax microplate readers. It provides centered transmission at 560 nanometers for measurements that require that wavelength and is supplied as an individual replacement lens.
- Optical center wavelength 560 nm.
- Compatible with EMax and VMax microplate readers.
- Sold as a single replacement filter lens.
- Intended for fluorescence and absorbance applications requiring a 560 nm bandpass.
Molecular Devices Fluorescence Polarization Filter, Polarized Horizontal, 300-750 nm
Molecular Devices polarized horizontal fluorescence polarization filter for use in fluorescence polarization assays on compatible multi-mode microplate readers. The filter transmits across a broad UV-visible range and installs in a standard filter slide to enable polarized detection.
- Polarized horizontal orientation.
- Wavelength range 300-750 nm.
- Enables fluorescence polarization measurements.
- Installs in a standard filter slide for compatible multi-mode readers.
- Broad UV-visible transmission for diverse fluorophores.
Molecular Devices Fluorescence Filter, 400 nm, 30 nm BW, Holder
This fluorescence filter with holder provides an optical band centered at 400 nm with a 30 nm bandwidth for use in microplate-based fluorescence assays, luminescence, and time-resolved fluorescence detection. It serves as an accessory optical element for multimode microplate readers.
- 400 nm center wavelength, 30 nm bandwidth
- Designed for fluorescence, luminescence, and time-resolved fluorescence detection
- Includes mounting holder for installation in standard filter slots
- Intended for use with multimode microplate readers and plate-based assays
Molecular Devices Polarization Filter, 700 nm BW 60 nm, Vertical
This fluorescence polarization filter in a holder enables near-infrared fluorescence polarization measurements centered at 700 nm and installs into the filter slot of compatible multi-mode microplate readers to support FP assays.
- 700 nm center wavelength with 60 nm bandwidth.
- Fluorescence polarization optical design for polarization-based measurements.
- Vertical polarization orientation for polarization detection.
- Mounted in a holder for insertion into reader filter slots.
- Intended for fluorescence polarization assays and near-IR detection workflows.
Molecular Devices Polarization Emission Filter, Visible, Pair, For FilterMax Readers
Replacement polarization emission filter set for fluorescence polarization measurements in multi-mode microplate readers. Supplied as a pair (quantity 2), these emission filters are intended to enable polarization-based fluorescence detection and improve signal discrimination in applicable assays.
- Replacement emission filters for fluorescence polarization assays.
- Visible emission band, vendor designation "Visible".
- Sold as a pair (quantity 2) for filter-based reader configurations.
- Intended for compatible multi-mode microplate readers.
- Helps improve polarization signal discrimination and assay reproducibility.
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