Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Enterprise Technology Solutions Customizable TOC Ready Index M
Customizable TOC Ready Index Multicolor Tab Dividers Uncollated 8-Tab 1 to 8 11 x 8.5 White 24 Sets
Electron Microscopy Sciences Nagoette Double Counting Cell 0.5 mm
Counting cells are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting. All complete with case and 1 pair of cover glasses. The depth of the Nageotte counting is 0.5 mm. For Liquids in Poor content.
Electron Microscopy Sciences USAF Test Chart R75N Negative Image
USAF resolution charts are recognized the world over as a universal standard for testing the vertical and horizontal resolution of imaging systems. Each element on the chart comprises three vertical bars and three horizontal bars, and the detail on these slides is as fine as 0.78microns (644 line-pairs per mm). The resolution of the imaging system is normally specified as the Group and Element of the finest bars that can be clearly defined. Sub Micron smallest feature size - bar width 0.00078mm [0.78 um].
Type: Negative Image
Use: Groups 0/1 to 9/3
Sigma Aldrich Fine Chemicals Biosciences PyrexR low actinic media s1L
Heavy-duty low actinic amber glass bottles protect light-sensitive materials. Include linerless one-piece autoclavable orange PP plug-seal caps (size GL 45) with drip-free pouring rings. Permanent white enamel graduations and marking spots.
Molecular Devices SpectraTest Validation Plate, FL1, Calibration Certified, Reader Compatible
The SpectraTest FL1 Fluorescence Validation Plate is a calibrated reference tool used to validate the optical performance of FL1-channel fluorescence-capable microplate readers. It verifies sensitivity, wavelength accuracy, RFU linearity, PMT matching, and other fluorescence performance parameters and ships with a certificate of calibration and protective storage.
- Validates fluorescence sensitivity, wavelength accuracy, PMT matching, and RFU linearity.
- Includes calibrated fluorescent and non-fluorescent reference strips and didymium glass.
- Supplied with a certificate of calibration and protective storage sleeve.
- Compatible with a wide range of microplate readers for FL1-channel testing.
- Recommended annual recertification to maintain traceable calibration.
Molecular Devices Luminescence Filter, 410 nm, BW 80 nm
This Molecular Devices luminescence emission filter (with holder) provides a 410 nm center wavelength with an 80 nm bandwidth for luminescence detection in microplate assays. It is listed as an accessory for SpectraMax iD series multi-mode microplate readers and is commonly used as a donor emission filter in assays such as BRET2.
- Provides a 410 nm center wavelength with an 80 nm bandwidth
- Includes holder for secure installation in filter slot
- Optimized for luminescence emission detection
- Used as donor emission filter in luminescence assays
- Compatible with multi-mode microplate reader filter systems
Revvity Health Sciences Inc Revvity HTRF Anti-6His mAb d2-Conjugate, 20,000 Assay Points, TR-FRET
This HTRF Anti-6His monoclonal antibody d2-Conjugate is a d2-labeled anti-6His antibody, specifically designed for capturing 6His-tagged proteins in protein/protein interaction assays.
- Designed for capturing 6His-tagged proteins.
- Used in protein/protein interaction assays.
- Features 20,000 assay points.
- Utilizes protein-protein interaction application.
- HTRF detection modality.
- Targets 6HIS.
- Binding assay target class.
- TR-FRET technology.
- Shipped ambient.
Alkali Scientific ASI™ Black Confocal Dish 35 x10mm, Easy-Grip, Adhesion Type, Sterile, Non-Treated (Non-Charged/ Hydrophobic), 100/Cs, 22 x 22 x 0.13mm, Hole 3.14mm, PS/Glass
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products:Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy Bottom materials: Glass/FLux (no surface treatment for glass bottom) Coverslip: #1.5 Dish color: Clear/Black Dish size: 35 Ø Hole size: 13 Ø / 20 Ø / 25Ø Non – pyrogenic Non – cytotoxic DNase / RNase – free Human DNA – free
Perkin Elmer US LLC Complete Capillary Injector Add On Kit for 120 V Clarus
Complete Capillary Injector Add On Kit for 120 V Clarus