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Loop-mediated isothermal amplification (LAMP) is a methodology for nucleic acid amplification that can be used as a diagnostic tool for disease detection. This process can be carried out without the need for a thermal cycler and may, thus, be cost effective. Suitable for the detection of COVID-19.
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The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes Loop-Mediated Isothermal Amplification (LAMP) to detect SARS-CoV-2 nucleic acid. The kit is available for research use only and includes WarmStart Colorimetric LAMP 2X Master Mix with UDG and a primer mix targeting the N and E regions of the viral genome. Controls are provided to verify assay performance, and include an internal control primer set and a positive control template. Guanidine hydrochloride has been found to increase the speed and sensitivity of the RT-LAMP reaction and is also included.
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