Loop-mediated isothermal amplification (LAMP) is a methodology for nucleic acid amplification that can be used as a diagnostic tool for disease detection. This process can be carried out without the need for a thermal cycler and may, thus, be cost effective. Suitable for the detection of COVID-19.
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Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2
The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.
|Includes||LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA|
|Detection Limit||Direct LAMP: <250 copies/25 μL LAMP reaction; Purified RNA-based LAMP: <100 copies/25 μL LAMP reaction|
|Bead Type||Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads|
|For Use With (Equipment)||Heating block|
|Enzyme||SuperScript IV & proprietary Bst-derived DNA polymerase|
|Formulation||Liquid. 5 components included in the kit.|
|Sample Type||Saliva or Nasopharyngeal/Nasal Swab|
|Color||During reaction color changes from purple to blue|
|Sample Volume||Range of sample inputs|
|Key Functions||Detects SARS-CoV-2 presence|
|For Use With (Application)||SARS-CoV-2 surveilance|
|Detection Method||Colorimetric readout|